DSpace university logo mark
Advanced Search
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > School of Pharmaceutical Sciences > Articles in academic journal >

Spleen-Selective Gene Transfer Following the Administration of Naked Plasmid DNA onto the Spleen Surface in Mice


File Description SizeFormat
bpb_30_941.pdf184.69 kBAdobe PDFView/Open

Title: Spleen-Selective Gene Transfer Following the Administration of Naked Plasmid DNA onto the Spleen Surface in Mice
Authors: Nakamura, Junzo / Fumoto, Shintaro / Kawanami, Rie / Kodama, Yukinobu / Nishi, Junya / Nakashima, Mikiro / Sasaki, Hitoshi / Nishida, Koyo
Issue Date: May-2007
Publisher: The Pharmaceutical Society of Japan
Citation: Biological & Pharmaceutical Bulletin v.30(5) p.941-945, 2007
Abstract: The purpose of present study was to examine spleen-selective gene transfer following the administration of naked plasmid DNA (pDNA) onto the spleen surface in mice. Gene expression in the spleen and other tissues was evaluated based on firefly luciferase activity. Six hours after spleen surface instillation of naked pDNA, high gene expression in the spleen was observed. On the contrary, intravenous and intraperitoneal administration of naked pDNA resulted in no detectable gene expression. After instilling naked pDNA onto the spleen surface, gene expression in the spleen was significantly higher than those in other tissues. Six hours after instillation of naked pDNA onto the spleen surface, gene expression in the spleen reached the peak value, and thereafter decreased gradually. By utilizing a glass-made diffusion cell that is able to limit the contact dimension between the spleen surface and naked pDNA solution administered, site-specific gene expression in the spleen was found. This novel gene transfer method is expected to be a safe and effective strategy for DNA vaccine against serious infectious diseases and cancers.
Keywords: gene therapy / plasmid DNA / spleen / transfection / mouse / luciferase
URI: http://hdl.handle.net/10069/15099
ISSN: 09186158
DOI: 10.1248/bpb.30.941
PubMed ID: 17473439
Relational Links: http://dx.doi.org/10.1248/bpb.30.941
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/15099

All items in NAOSITE are protected by copyright, with all rights reserved.

 

Valid XHTML 1.0! Copyright © 2006-2015 Nagasaki University Library - Feedback Powerd by DSpace