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Adsorption Chromatography of Dinitrophenylamino Acids on Silica Gel-Celite


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Title: Adsorption Chromatography of Dinitrophenylamino Acids on Silica Gel-Celite
Authors: Matsuda, Genji / Maita, Tetsuo / Takei, Hiroshi / Maekawa, Tomoyuki / Shikaya, Takao / Maeda, Kenji / Fujiwara, Masaroku
Authors (alternative): 松田, 源治 / 毎田, 徹夫 / 武居, 洋 / 前川, 知之 / 鹿谷, 隆朗 / 前田, 謙而 / 藤原, 正六
Issue Date: 25-Dec-1963
Citation: Acta medica Nagasakiensia. 1963, 8(1-2), p.28-34
Abstract: The technique of adsorption chromatography of dinitrophenylamino acids (DNP-amino acids) has been studied in this laboratory in order to separate N-terminal amino acids from the globins of various hemoglobins. The following method which gave good results is a modified method of GREEN et al. A mixture of silica gel and celite (2 to 1) is packed into a 15-cm. column with diameter of 0.9 cm. As developers mixtures of organic solvents such as acetic acid, acetone, ligroin, formic acid, ethyl acetate or benzene are used. In the above column and with the above developers, adsorption chromatography is carried out to separate various DNP-amino acids. This seems to be an effective method for qualitative and quantitative analysis of DNA-amino acids.
URI: http://hdl.handle.net/10069/15475
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 8, No. 1-2

Citable URI : http://hdl.handle.net/10069/15475

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