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Studies on Anaphylactic Manifestation in Guinea Pig's Atria by Means of Albumin Labeled with Fluorescein

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Title: Studies on Anaphylactic Manifestation in Guinea Pig's Atria by Means of Albumin Labeled with Fluorescein
Authors: Fujita, Tsutomu
Authors (alternative): 藤田, 勉
Issue Date: 25-Mar-1965
Citation: Acta medica Nagasakiensia. 1965, 9(3-4), p.212-221
Abstract: It has been widely accepted that the anaphylactic reaction might be caused principally by chemical substances released from the tissues as a result of antigen-antibody reaction. Atria isolated from guinea pigs sensitized by egg-albumin were mainly used for this experiment. When the sensitized atrium and the non-sensitized guinea-pig's ileum were suspended together in the same bath, there appeared strong contraction of the ileum along with the provocation of anaphylaxis in the sensitized atrium. Although such contraction of ileum was abolished almost completely by diphenhydramine or diphenylpyraline, the atrial anaphylaxis was not affected. The anaphylactic reaction was also evoked by perfusing solution containing the antigen. However, the atrial anaphylaxis showed little influence on the contractile tension of the non-sensitized atrium suspended together in the same bath. The above facts suggest that the anaphylactic contraction would not be caused solely by mediators, including hitherto unknown chemical substances. So the further attempt was carried out to examine in vitro the site of antigen-antibody interaction in the atrium by using the antigen labeled with fluorescein isothiocyanate. The specific fluorescence about the muscle cells was observed along their boundary of the highly sensitized atrium. Through the results in these experiments, together with the reports from our laboratory, it could be assumed that in the atrial anaphylaxis the antigen-antibody reaction itself induces some changes on the cell membrane leading to muscular contraction.
URI: http://hdl.handle.net/10069/15495
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 9, No. 3-4

Citable URI : http://hdl.handle.net/10069/15495

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