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A Study on the α- β-and γ-Polypeptide Chains in Normal Human Adult and Fetal Hemoglobin by Cellulose Acetate Membrane Electrophoresis


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Title: A Study on the α- β-and γ-Polypeptide Chains in Normal Human Adult and Fetal Hemoglobin by Cellulose Acetate Membrane Electrophoresis
Authors: Kobayashi, Shinnichi
Authors (alternative): 小林, 信一
Issue Date: 25-Oct-1965
Citation: Acta medica Nagasakiensia. 1965, 10(1-2), p.12-28
Abstract: For the purpose of separation and identification of the α-, β-and γ-polypeptide chains contained in healthy human adult and fetal hemoglobins, studies were performed by means of cellulose acetate membrane electrophoresis. On this method of electrophoresis, a comparative examination was made in various combinations of the effects of the urea concentration, temperature and time in the treatment of the globins before electrophoresis, and the effects of the urea concentration, ionic strength, and pH of the veronal buffer in course of electrophoresis. As the result, this method proved to be a simple and rapid way to separate the α-, β-and γ-polypeptide chains of human hemoglobin with an efficient ability of separation. For a better separation of these three kinds of polypeptide chains, desired results were recognized when the globin was incubated in 8 M urea solution at a room temperature for 30 min for the pretreatment before electrophoresis, and was subjected to electrophoresis at a current of 0.5 mA/cm for 30 min by the use of a veronal buffer of ionic strength 0.045 and pH 6.2- 6.5 containing 6 M urea.
URI: http://hdl.handle.net/10069/15499
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 10, No. 1-2

Citable URI : http://hdl.handle.net/10069/15499

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