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Amino Acid Sequence of the Insoluble Tryptic Peptide,αT12,13 from theα-Polypeptide Chain in Macaca mulatta Monkey Hemoglobin


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タイトル: Amino Acid Sequence of the Insoluble Tryptic Peptide,αT12,13 from theα-Polypeptide Chain in Macaca mulatta Monkey Hemoglobin
著者: Ota, Hisahiro
著者(別表記) : 大田, 尚弘
発行日: 1968年10月25日
引用: Acta medica Nagasakiensia. 1968, 13(1-2), p.30-43
抄録: Amino acid sequence analysis was carried out on the so-called 'insoluble tryptic peptide(αT12,13)'which was precipitated when the tryptic hydrolysate of the α-polypeptide chain in macaca mulatta monkey hemoglobin was adjusted to pH 6.4. This peptide was oxidized with performic acid and then was hydrolyzed with chymotrypsin. The chymotryptic hydrolysate was isolated by column chromatography, purified by paper chromatography, and amino acid compositions of the peptides in the hydrolysate were analyzed. Amino acid sequences of these chymotryptic peptides were determined by the DNP and PTC methods and the partial hydrolysis. In addition, the oxidized insoluble tryptic peptide was rehydrolyzed in 1N NH4OH at 0℃ with trypsin for 7 hours. Amino acid compositions and N-terminal amino acid residues of the peptides isolated and purified by column and paper chromatographies were examined. By considering these results together, the amino acid sequence of the insoluble tryptic peptide(αT12,13)was determined. It was compared with the known sequence of human hemoglobin.
URI: http://hdl.handle.net/10069/15536
ISSN: 00016055
資料タイプ: Departmental Bulletin Paper
原稿種類: publisher
出現コレクション:Volume 13, No. 1-2

引用URI : http://hdl.handle.net/10069/15536

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