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Amino Acid Sequence of the Insoluble Tryptic Peptide,αT12,13 from theα-Polypeptide Chain in Macaca mulatta Monkey Hemoglobin


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Title: Amino Acid Sequence of the Insoluble Tryptic Peptide,αT12,13 from theα-Polypeptide Chain in Macaca mulatta Monkey Hemoglobin
Authors: Ota, Hisahiro
Authors (alternative): 大田, 尚弘
Issue Date: 25-Oct-1968
Citation: Acta medica Nagasakiensia. 1968, 13(1-2), p.30-43
Abstract: Amino acid sequence analysis was carried out on the so-called 'insoluble tryptic peptide(αT12,13)'which was precipitated when the tryptic hydrolysate of the α-polypeptide chain in macaca mulatta monkey hemoglobin was adjusted to pH 6.4. This peptide was oxidized with performic acid and then was hydrolyzed with chymotrypsin. The chymotryptic hydrolysate was isolated by column chromatography, purified by paper chromatography, and amino acid compositions of the peptides in the hydrolysate were analyzed. Amino acid sequences of these chymotryptic peptides were determined by the DNP and PTC methods and the partial hydrolysis. In addition, the oxidized insoluble tryptic peptide was rehydrolyzed in 1N NH4OH at 0℃ with trypsin for 7 hours. Amino acid compositions and N-terminal amino acid residues of the peptides isolated and purified by column and paper chromatographies were examined. By considering these results together, the amino acid sequence of the insoluble tryptic peptide(αT12,13)was determined. It was compared with the known sequence of human hemoglobin.
URI: http://hdl.handle.net/10069/15536
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 13, No. 1-2

Citable URI : http://hdl.handle.net/10069/15536

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