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The Amino Acid Sequence of the Insoluble Tryptic Peptide,βT10,11,12 from theβ-Polypeptide Chain in Macaca mulatta Monkey Hemoglobin


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タイトル: The Amino Acid Sequence of the Insoluble Tryptic Peptide,βT10,11,12 from theβ-Polypeptide Chain in Macaca mulatta Monkey Hemoglobin
著者: Yamaguchi, Masato
著者(別表記) : 山口, 正人
発行日: 1968年10月25日
引用: Acta medica Nagasakiensia. 1968, 13(1-2), p.67-84
抄録: Globin prepared from macaca mulatta monkey hemoglobin was separated into α and β-polypeptide chains by countercurrent distribution method. The β-polypeptide chain was digested with trypsin. The insoluble tryptic peptide (so-called β-core), the precipitate of the digest at pH 6.4, was obtained by centrifugation. This insoluble tryptic peptide after oxidized with performic acid was digested with pepsin or redigested with trypsin, and the amino acid composition was examined. Next, the amino acid sequence of the β-core containing 38 amino acid residues was determined by the DNP method, the PTC method, and the carboxypeptidase method. The result was compared with the known sequence of the β-core of human hemoglobin. Between these two hemoglobins, two amino acid substitutions were found; that is, the 5th and 22nd residues from the N-terminus are threonine and arginine in case of human hemoglobin, but glutamine and lysine, respectively in case of macaca mulatta monkey hemoglobin.
URI: http://hdl.handle.net/10069/15538
ISSN: 00016055
資料タイプ: Departmental Bulletin Paper
原稿種類: publisher
出現コレクション:Volume 13, No. 1-2

引用URI : http://hdl.handle.net/10069/15538

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