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Isolation of All Tryptic Peptides from the Aminoethylated β Polypeptide Chain of Adult Hemoglobin from Japanese Monkey (Macaca fuscata fuscata) and their Amino Acid Compositions


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Title: Isolation of All Tryptic Peptides from the Aminoethylated β Polypeptide Chain of Adult Hemoglobin from Japanese Monkey (Macaca fuscata fuscata) and their Amino Acid Compositions
Authors: Tachikawa, Isamu
Authors (alternative): 立川, 勇
Issue Date: 25-Mar-1969
Citation: Acta medica Nagasakiensia. 1969, 13(3-4), p.157-169
Abstract: Hemoglobin prepared from red blood cells of adult Japanese Monkeys (Macaca fuscata fuscata) was removed heme so that globin was obtained. The globin was separated into α and β polypeptide chains by countercurrent distribution with the system of sec-butanol containing trichroloacetic acid, propionic acid, and water. The,β polypeptide chain was aminoethylated and hydrolyzed with trypsin. The tryptic peptides were isolated and purified by column chromatography, paper chromatography, and high voltage paper electrophorsis. Analyses of amino acid compositions of these peptides were performed after HCl hydrolysis. In comparison with human hemoglobin A, Japanese monkey hemoglobin turned out to be different at at least seven positions of the five peptides from the β polypeptide chain. Similarly, in comparison with rhesus monkey hemoglobin, only one difference in the amino acid composition could be found between rhesus monkey (macaca mulatta) and Japanese monkey which belong to the common genus.
URI: http://hdl.handle.net/10069/15545
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 13, No. 3-4

Citable URI : http://hdl.handle.net/10069/15545

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