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Kinetic Changes of DNA and RNA Contents in Activated Lymphocytes upon Lectin Stimulation and Operative Insult


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Title: Kinetic Changes of DNA and RNA Contents in Activated Lymphocytes upon Lectin Stimulation and Operative Insult
Authors: Ishibashi, Tsunehisa
Authors (alternative): 石橋, 経久
Issue Date: 25-Oct-1985
Citation: Acta medica Nagasakiensia. 1985, 30(1-3), p.225-237
Abstract: The cellular DNA and RNA contents of the lymphocytes were assessed by using a Fluorescence Activating Cell Sortor System (FACS-IV)to evaluate the lymphocyte response to the lectin stimulation or the operative insult . 1) The DNA and RNA contents in the lymphocytes stimulated by the lectins such as phytohemagglutinin (PHA), Concanavalin A (Con A) and Pokeweed mitogen (PWM) were measured in vitro by using the technique of FACS-IV in comparision with those by the uptakes of 3H-thymidine dR and Uridine R . The DNA and RNA syntheses began to start at the first 6 hours of stimulation by the lectins in accordance with an appearance of the lymphocyte in early S phase and it was apparent that mitosis occurred for a period between 36 and 48 hours . It is to be further emphasized that DNA syntheses in the lectin-stimulated lymphocytes were initiated with the passage of 6 hours and remarkably increasing as those at 12 and 24 hr . 2) Postoperative states were evaluated from the standpoint of change in the cellular RNA contents in the monocytes. An increase in the RNA content was noticeable on day 1 to day 7 following surgery and these changes were associated with postoperatively serious conditions such as either grave complication or major operative invasion . In conclusion, the assessment of the cellular RNA content in the monocytes plays an important role in identifying postoperative state from serious condition on the course of postoperative period.
URI: http://hdl.handle.net/10069/15681
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 30, No. 1-3

Citable URI : http://hdl.handle.net/10069/15681

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