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Legionnaires' Disease, its Bacteriological Characteristics, Distribution and Clinical Features in Japan


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Title: Legionnaires' Disease, its Bacteriological Characteristics, Distribution and Clinical Features in Japan
Authors: Hara, Kohei / Saito, Atsushi
Issue Date: 20-Mar-1986
Citation: Acta medica Nagasakiensia. 1986, 30(4), p.321-324
Abstract: DISTRIBUTION OF LEGIONELLA SPECIES FROM ENVIRONMENTAL SOURCE IN JAPAN Each 500 ml of 408 water samples including 343 coiling tower water from sites widely distributed over Japan, 25 samples of paddy field, 29 samples of river and 11 samples of lake, fountain, puddle and shower water in Nagasaki area, was obtained and centrifuged at 10,000 rpm for 20 min. The sediments were cultured in a selective medium1) for isolation of Legionella spp. after low pH treatment. In some negative samples, 3 ml of the sediments were inoculated to guinea pigs intraperitoneally and they were sacrificed four days later. The peritoneal swab and homogenate of spleen were inoculated to B-CYE agar, Each 200 g of soil was suspended in 400 ml of distilled water with 0.5% of Tween 60 far 20 min. The supernatant was centrifuged at 1,000 rpm for 10 min., and again the supernatant was centrifuged at 3,000 rpm for 10 min. The sediment were obtained and used for isolation of Legionella spp.
URI: http://hdl.handle.net/10069/15698
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 30, No. 4

Citable URI : http://hdl.handle.net/10069/15698

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