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Transcriptional modulation of a flanking gene by HTLV-1 integration, and demonstration of a cellular transcript homologous to HTLV-1 LTR-gag region


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Title: Transcriptional modulation of a flanking gene by HTLV-1 integration, and demonstration of a cellular transcript homologous to HTLV-1 LTR-gag region
Authors: Moriuchi, Ryozo / Igarashi, Hisanaga / Nakayama, Daisuke / Miyamoto, Tsutomu / Hino, Shigeo
Issue Date: 25-Oct-1988
Citation: Acta medica Nagasakiensia. 1988, 33(1-4), p.38-47
Abstract: We have cloned a human T-lymphotropic virus type-1(HTLV-1 ) proviral genome, λ 255 M, of a cell line, TL-Su, which has been established from peripheral blood lymphocytes of a healthy HTLV-1 carrier. The λ 255 M had a very similar restriction map to that of a previous isolate, λ ATK-1, but a different junctional repeat, TGAAAG, consistent with the random integration sites of HTLV-1. We found two species of interesting cellular gene transcripts. The first gene homologous to LTR-gag region of HTLV-1 was expressed as 4.5 kb RNA only in the cells of lymphoid cell lineage as far as tested. The RNA was present even in uninfected cells including a T-cell line, Jurkat, and a B-cell line, FLEB-12-3-4, but not in another T-cell line, CEM. Because of strong viral RNA signals, we could not demonstrate the RNA in virus producing cell lines. The results suggested the presence of human cellular gene related with LTR-gag region of HTLV-1. The other gene homologous to 5' flanking gelle of the λ 255 M was expressed as 1.8kb mRNA in all human cells tested except for the TL-Su cell. In the TL-Su cell, HTLV-1 integration modulated this gene transcription into 3.8, 3.2. 2.0 and 1.6kb mRNAs qualitatively, and at least 10 times more than other human cells quantitatively. The data suggests that HTLV-1 gene integration may cause the transcriptional modulation of cellular genes by insertional mutagenesis. (key words, HTLV-1; HTLV-1 related human gene; transcriptional modulation; flanking sequence; insertional mutagenesis )
URI: http://hdl.handle.net/10069/15705
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 33, No. 1-4

Citable URI : http://hdl.handle.net/10069/15705

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