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Local Immunological Defense of the Tumor-bearing Host in Abdominal Surgery with Special Reference to Peritoneal Macrophage-Mediated Cytostasis

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Title: Local Immunological Defense of the Tumor-bearing Host in Abdominal Surgery with Special Reference to Peritoneal Macrophage-Mediated Cytostasis
Authors: Oh, Shimei
Issue Date: 25-Oct-1988
Citation: Acta medica Nagasakiensia. 1988, 33(1-4), p.208-220
Abstract: There were reported some kinds of anticancer drugs which have immunopotentiating effect. In this study, we demonstrated the i.p injection of CDDP which enhance the macrophage-mediated cytostatic effect in mice. Flow cytometry has been used to study the influence of CDDP-activated macrophages on the cell cycle of Meth-A cells, and the biochemical changes of the CDDP-activated macrophages were analysed. Macrophages harvested four days later from mice treated with i.p injection of CDDP 0.3-4 mg/kg showed a dose-dependent effect to both EL-4 and Mrth-A cells. The activation was mainly seen in the plastic adhesive cells and was diminished by treating with carrageenan. In vivo Winn assay, CDDP-activated macrophages showed a significant inhibitory effect to the proliferation of Meth-A cells for at least 15 days. The survival time was elongated when the tumor-bearing mice were treated with i.p injection of CDDP. However, the elongative effect was diminished by the pretreatment of carragenan. On the other hand, Flow cytometry analysis using Propidium Iodide staining was designed to study the mechanism of the tumor cell inhibitory effect by CDDP and CDDP-activated macrophages. Changes of cell elements such as RNA, protein, and mitochondria were evaluated by A.O, FITC and Rhodamin 123 staining technique respectively. CDDP does enhance the antitumor effect of the peritoneal macrophages of mice. Using the Flow cytometry analysis we have been able to demonstrate that in contrast to CDDP which accumulate the Meth-A cells in the G2M phase of the cell cycle, CDDP-activated macrophages appeared to have a different influence more restricted to the G1/S block of the cycle. CDDP-activated macrophages resembled to OK-432-activated macrophages, showing an increase of RNA, protein synthesis and an enhancement of the mitochondria menbrane potential.
URI: http://hdl.handle.net/10069/15745
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 33, No. 1-4

Citable URI : http://hdl.handle.net/10069/15745

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