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Molecular Cloning of the Japanese Quail α A Globin cDNA


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Title: Molecular Cloning of the Japanese Quail α A Globin cDNA
Authors: Oshiro, Minoru / Eguchi, Yukinori / Toda, Takayoshi / Takei, Hiroshi / Nakashima, Yasutsugu
Issue Date: 9-Dec-1989
Citation: Acta medica Nagasakiensia. 1989, 34(2-4), p.18-23
Abstract: Double stranded cDNA was synthesized using the poly (A) + RNA of the Japanese quail reticulocytes and ligated into the EcoRI site ofλgt 10 phage DNA. A recombinant phage,λQαG1 was selected by plaque hybridization using a 30mer synthetic oligonucleotide probe specific to the α globin gene. The size of the cDNA insert in the recombinant phage DNA was 0.53 kb and the restriction map was similar to that of the chicken α A globin gene. Nucleotide sequencing of the cDNA insert indicated that it contained the entire coding information for the α A globin.
URI: http://hdl.handle.net/10069/15756
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 34, No. 2-4

Citable URI : http://hdl.handle.net/10069/15756

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