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Effect of Hyperthermia with Interferon on Malignant Cell Growth and Cell Cycle

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Title: Effect of Hyperthermia with Interferon on Malignant Cell Growth and Cell Cycle
Authors: Satou, Tetuya
Issue Date: 9-Dec-1989
Citation: Acta medica Nagasakiensia. 1989, 34(2-4), p.191-198
Abstract: Combined therapy of hyperthermia with interferon-β (rHuIFN-β) for carcinoma using HeLa cells was evaluated by in vitro study. Inhibitory effect on cell growth curve was observed in thetreatment of rHuIFN-β and/or hyperthermia alone. On the other hand, combined treatment much more promoted the inhibitory effect rather than single method. From the standpoint of the cell cycle on the basis of a result of flow cytometry study, a reduction of the G1-phase cells population and accumulation of the S-phase cells were significantly noted. Furthermore, the addition of hyperthermia significan t-ly recruited the S-phase cells. When administering rHuIFN-β, the activity of DNA polymerase α was reduced and that of DNA polymerase β was inversely increased. While hyperthermia was added, both the levels of DNA polymerise α and β activities were decreased. It was different patterns in terms of enzymatic activities regarding accumulation of the S-phase cells. In conclusion, in order to get the cytocidal effect on the HeLa cells, the ideal timing applying combined treatment of HuIFN-β with hyperthermia is that HuIFN-β should be first given, thereafter hyperthermia is applied so as to adjust to the S-phas e of the cell cycle which is greatly sensitive to hyperthermia.
URI: http://hdl.handle.net/10069/15786
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 34, No. 2-4

Citable URI : http://hdl.handle.net/10069/15786

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