DSpace university logo mark
Advanced Search
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > School of Medicine > Bulletin > Acta Medica Nagasakiensia > Volume 35, No. 1-4 >

In Vitro Study of the Independent and Combined Effects of Recombinant Human GM-CSF and G-CSF on Normal Bone Marrow Granulocytes : GM-CSF Enhances the Growth Effect but Suppresses the Terminal Maturation-inducing Effect of G-CSF


File Description SizeFormat
acta35_01_13_t.pdf321.21 kBAdobe PDFView/Open

Title: In Vitro Study of the Independent and Combined Effects of Recombinant Human GM-CSF and G-CSF on Normal Bone Marrow Granulocytes : GM-CSF Enhances the Growth Effect but Suppresses the Terminal Maturation-inducing Effect of G-CSF
Authors: Matsuo, Tatsuki / Tomonaga, Masao / Jinnai, Itsuro / Kuriyama, Kazutaka / Amenomori, Tatsuhiko / Yoshida, Yoshiharu / Ichimaru, Michito / Golde, Davidw.
Issue Date: 14-Dec-1990
Citation: Acta medica Nagasakiensia. 1990, 35(1-4), p.63-66
Abstract: We studied the independent and combined effects of recombinant human granulocyte colony- stimularting factor (rhG-CSF) and recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) on the growth and maturation of neutrophilic granulocytes. Absolute granulocyte numbers, cellular composition, and neutrophil alkailne phosphatase (NAP) activity were examined after normal nonphagocytic bone marrow cells were cultured for seven days with rhG-CSF, rhGM-CSF, and a combination of both growth factors. The numbers of band and segmented forms produced in the cultures containing rhGM-CSF were significantly lower than in control cultures or those with rhG-CSF. When both rhGM-CSF and rhG-CSF were in the culture total granulocyte production was highest, but the increment of band and segmented forms produced by rhG-CSF alone was reduced. Neutrophil alkaline phosphatase (NAP), an enzyme marking terminal maturation, was increased by rhG-CSF alone, but not by rhGM-CSF alone. Cultures containing both CSFs showed significantly lowered NAP activity compared to those containing rhG-CSF alone. The decrement in NAP activity was proportionate to the amount of rhGM-CSF added. These results indicate that terminal maturation-inducing effect is a property of rhG-CSF but not of rhGM-CSF. In the presence of both GM-CSF and G-CSF, the growth of granulocytes is maximumly stimulated but the terminal maturation-inducing effect of rhG-CSF is suppressed.
URI: http://hdl.handle.net/10069/15813
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 35, No. 1-4

Citable URI : http://hdl.handle.net/10069/15813

All items in NAOSITE are protected by copyright, with all rights reserved.

 

Valid XHTML 1.0! Copyright © 2006-2015 Nagasaki University Library - Feedback Powerd by DSpace