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A Study on Enhancement of Hyperthermochemotherapy in the Presence of Buthionine Sulfoximine (BSO)


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Title: A Study on Enhancement of Hyperthermochemotherapy in the Presence of Buthionine Sulfoximine (BSO)
Authors: Kunisaki, Tadaomi
Issue Date: 14-Dec-1990
Citation: Acta medica Nagasakiensia. 1990, 35(1-4), p.209-215
Abstract: The aim of this study was enhancement of the effect of hyperthermia which is a part of the multidisciplinary treatments for carcinomas. Many physicians suffered from practical difficulty in keeping the uniformity of 43℃ in the deep organs. Buthionine sulfoximine (BSO) is a specific inhibitor of glutathione (GSH) synthesis, and suppress GSH activity which is related to the repair process of cell damage. In this study, colony formation was tested using HeLa cells on the condition of one hour heating in the presence of 1mM BSO which is suitable for clinical application. The concentration of 0.08ug cis DDP was used, corresponding to LD25. Colony formation was reduced to 45% and 19% at 40℃ and 41℃ in combination cis DDP in the presence of BSO. From the analysis of flowcytometry inhibition of accumulation in G2M phase was observed at 12 and 24 hours exposing to 41℃ and 42℃ heating in the presence of BSO. The cells were increased in number for 24 hours at 41ーC heating on the basis of analysis of cell growth curve. Atotal of GSH were reduced to 2.4% in the presence of BSO as compared with 41℃ heating alone. It is concluded that BSO helps escape repair process from thermal cytotoxicity and BSO plays an important role in promoting the effect of chemohyperthermia for the treatment, of carcinomas.
URI: http://hdl.handle.net/10069/15843
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 35, No. 1-4

Citable URI : http://hdl.handle.net/10069/15843

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