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Receptor Autoratiographic Evidence for Specific 125I-Endothelin-1 Binding Sites in the Rat Eye


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タイトル: Receptor Autoratiographic Evidence for Specific 125I-Endothelin-1 Binding Sites in the Rat Eye
著者: Tashiro, Junko
発行日: 1993年12月25日
引用: Acta medica Nagasakiensia. 1993, 38(2-4), p.86-90
抄録: Specific 125I-endothelin-1 (125I-ET-1) binding sites were investigated in the rat eye, using receptor autoradiographic techniques. 125I-ET-1 was specifically bound to the rat eye sections, with a slow association rate, as evidenced by kinetic experiments. The radioligand binding reached a maximum at 48hr of incubation and a plateau was maintained for up to 72hr. No degradation of 125I-ET-1 during incubation was observed at 72hr. Specific 125I-ET-1 binding sites were localized in areas corresponding anatomically to the cornea, the iris, the retina, the choroid and the sclera. 125I-ET-1 binding to these sections was monophasically inhibited by unlabeled ET-1 with dissociation constant (Kd) of 128pM, whereas unlabeled ET-3, a member of the ET family peptides, biphasically inhibited binding with low affinity, inhibition constant (Ki) of 7.06nM, and high affinity, Ki of 53pM. This evidence for specific 125I-ET-1 binding sites supports the physiological significance of the ET family peptides in the rat eye.
キーワード: spontaneous haemopneumothorax / thoracotomy / decortication / pleural adhesion
URI: http://hdl.handle.net/10069/15914
ISSN: 00016055
資料タイプ: Departmental Bulletin Paper
原稿種類: publisher
出現コレクション:Volume 38, No. 2-4

引用URI : http://hdl.handle.net/10069/15914

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