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Ultrastructural changes of the mitochondria in autotransplanted canine pancreas after cold storage for 72 an 96 hours


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Title: Ultrastructural changes of the mitochondria in autotransplanted canine pancreas after cold storage for 72 an 96 hours
Authors: Arita, Satoshi
Issue Date: 25-Dec-1993
Citation: Acta medica Nagasakiensia. 1993, 38(2-4), p.124-129
Abstract: To investigate the limit of safe cold storage of the pancreas, the left lobe of the pancreas was stored in University of Wisconsin solution for 72 or 96 hours at 4℃ and then autotransplanted in 10 mongrel dogs. Ultrastructural changes of the mitochondria in islet B cells, acinar cells, and endothelial cells were studied. After storage for 72 hours, the mitochondria of islet B cells were well preserved. After 96 hours, however, mitochondrial swelling was evident as well as a decrease in the number of cristae. These changes were more severe in acinar cells than in islet cells, but they were normalized in both types of cells within 6 hours of autotransplantation. Three of the 5 grafts stored for 96 hours showed long-term function and maintained a normal fasting plasma glucose level. However, impairment of glucose tolerance following intravenous glucose loading was significant when compared to the 72 hours storage group. Three grafts stored for 96 hours failed to function due to arterial thrombosis, and degeneration and necrosis of the islet endothelial cells was demonstrated by electoron microscopy. This endothelial cell damage was possibly associated with impaired glucose tolerance and arterial thrombosis in the 96 hours group. These findings suggest that pancreatic storage for 72 hours has little effect on graft viability or function, while storage for 96 hours causes definite impairment.
Keywords: Canine pancreatic transplantation / University of Wisconsin / solution / Pancreatic preservation / Mitochondria
URI: http://hdl.handle.net/10069/15922
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 38, No. 2-4

Citable URI : http://hdl.handle.net/10069/15922

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