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Heat Shock Protein in the Analysis of Cell Kinetics in the Cell Cycle


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Title: Heat Shock Protein in the Analysis of Cell Kinetics in the Cell Cycle
Authors: Fukukawa, Taizou
Issue Date: 25-Dec-1993
Citation: Acta medica Nagasakiensia. 1993, 38(2-4), p.132-137
Abstract: Hyperthermia at 43℃ for 60 minutes was added in FM3A cells to elucidate the effect of heating on cells. 1) Accumulation in the G2M phase was noted 12 and 24 hours after heating in the analysis of the cell cycle by using flow cytometry. 2) The disturbed cell cycles by heating returned to the normal cycle 48 hours after heating. 3) The intracellular protein content increased in the G2M phase 12 to 24 hours after heating at 43℃ for 60min in spite of no remarkable changes in the G1 and S phases. 4) It is suggestive of an increase in heat-induced protein within cells in the G2M phase In conclusion, the intracellular protein increased in the G2M phase, suggesting a presence of HSP70 in analysis of SDS-polyacrylamide gel electrophoresis.
URI: http://hdl.handle.net/10069/15924
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 38, No. 2-4

Citable URI : http://hdl.handle.net/10069/15924

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