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Efficacy of Modified University of Wisconsin Solution for 24-Hour Preserved Canine Lung


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Title: Efficacy of Modified University of Wisconsin Solution for 24-Hour Preserved Canine Lung
Authors: Sasaki, Nobufumi
Issue Date: 25-Oct-1994
Citation: Acta medica Nagasakiensia. 1994, 39(1-3), p.106-112
Abstract: A 24-hours cold preserved lung using a low potassium UW solution (4 mEq/L or 30 mEq/L) was experimentally allotransplanted on dogs and subsequently the grafts function was carefully evaluated. Twenty-three adult mongrel dogs underwent thoracotomy by median sternotomy and pulmonary function was envaluated after the right bronchus and the right pulmonary artery were clamped. And then the both lungs and heart were flashed through the pulmonary artery with 50 ml/kg of modified UW solution at a pressure of 10-14 mmHg ; Group 1 (n=7), grafted lungs were flushed and preserved with original UW solution ([K^+ ]=120 mEq/L); Group 2 (n=10), a donor lungs were flushed and preserved with modified UW solution ([K^+ ]=30 mEq/L); Group 3 (n=6), the lungs were preserved with extracellular type UW solution ([K^+]=120 mEq/L) after flushing. The left lung was allografted after 24-hour cold preservation. During storage, the lung was inflated. Graft function was evaluated one hour after reperfusion and prior to sacrifice. The dogs ware sacrificed between 4 days and 4 weeks after transplanation. Mean cold ischemia and warm ischemia times were 24.2±0.4 hours and 71.5 ± 14.2 min, respectively. Flushing time was longer in Group 1 (287.3±107.0 sec) than in any other group (160.0±107.0 sec in Group 2, 163.2±58.3 sec in Group 3), but there was no significant difference among the groups. Two of the 7 dogs in Group 1, 2 of the 10 dogs in Group 2 and 4 of the 6 dogs in Group 3 did not survive more than 3 days. The Pa 02, lung water content, and dynamic compliance showed no significant difference among the groups. The static compliance at one hour after reperfusion was 15.43±2.88 ml/cmH2O in Group 1, 19.46±3.12 ml/cmH2O in Group 2 and 17.21±3.12 ml/cmH2O in Group 3. The Cst at one hour after reperfusion was higher in Group 2 than in Group 1 (P<0.05). The PVR increased to 2339.9±623.3 dyne . sec . cm^<-5> in Group 1, 1365.3 ±482.3 dyne . sec . cm^<-5> in Group 2 and 2051.1±278.2 dyne sec . cm^<-5> in Group 3. The PVR was lower in Group 2 than in Group 1 (P<0.05) at one hour after reperfusion. In conclusion, the modified UW solution containig the potassium concentration of 30 mEq/L is suitable to the original UW solution ([K^+]=120 mEq/L) or extracellular type UW solution ([K^+]=4 mEq/L) for the 24-hour cold preservation of canine lung allografts.
URI: http://hdl.handle.net/10069/15981
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 39, No. 1-3

Citable URI : http://hdl.handle.net/10069/15981

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