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Efficacy of a Platelet-Activating Factor Antagonist TCV-309 for Lung Preservation


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Title: Efficacy of a Platelet-Activating Factor Antagonist TCV-309 for Lung Preservation
Authors: Tagawa, Tsutomu
Issue Date: 25-Oct-1994
Citation: Acta medica Nagasakiensia. 1994, 39(1-3), p.113-118
Abstract: To evaluate the efficacy of a platelet-activating factor (PAF) antagonist, TCV-309, for the prevention of lung reperfusion injury, an experimental study was performed by using a canine isolated lung reperfusion model. The canine lungs were preserved for 24 hours at 4°C. The animals were classified into 4 groups: Group A, domor lungs were flushed with a Euro-Collins (EC) solution only and preserved for 24 hours at 4°C as controls (n=7) ; Group B, donor dogs received intravenous TCV-309 at a dose of 30 μg/kg 15 minutes before heparin sodium administration, which was given prior to isolating the lungs and donor lungs were preserved for 24 hours at 4°C (n= 8); Group C, donor lungs were flushed with a EC solution containing TCV-309 (375 μg/500m1) and preserved for 24 hours at 4°C (n= 6), Group D, donor lungs were flushed and stored with a EC solution and then reperfused with allogeneous blood containing TCV-309, 0.75 μ g/ml of blood volume (n= 6). The pulmonary vascular resistance (mmHg/l/min) was significantly lower for Groups B, C and D than for Group A (A vs. B or C or D: 270. 8± 111.3 vs. 96.2± 78.3 (p <0.01) or 122.6± 75.4 (p <0.05) or 120. 3± 89.7 (p <0.05), respectively) (120 min). The airway pressure (AWP, cmH2O) was significantly lower for Groups B and C than for Group A (A vs. B or C or D: 21.0 ± 3.3 vs. 16.0 ± 2.2 (p <0.01) or 15.7 ± 2.2 (p <0.01) or 18.3 ± 1.4 (N.S.), respectively) (0 min). The AWP was significantly lower for Group D than for Group A (A vs. B or C or D: 30.4± 8.6 vs. 2 3.4 ± 7.0 (N.S.) or 21.5 ± 7.8 (N.S.) or 19.1 ± 2.0 (p < 0.01), respectively) (120 min). The static lung compliance (ml/cm H20) was significantly higher for Groups B, C and D than for Group A throughout the reperfusion. The blood oxygen tension(ΔPO2, mmHg)and lung water(%)were signifi一 cantly improved for Groups C and D, but not for Group A (ΔPO2, Avs.BorCorD:161.1ア66.2vs.246.7ア82.4(N.S.)or 309.9± 62.2 (p <0.05) or 307.2± 79.0 (p <0.05), lung water; 89.6 ± 0.8 vs. 87.3± 2.6 (N.S.) or 86.0 ± 1.7 (p < 0.0001) or 85.2 ± 2.3 (p <0.005). At the end of the harvest, the tissue level of lipid peroxidation (nmolMDA/mg protein) was significantly lower for Group B than for the others (B vs. others: 1.17 ± 0.2 3 vs. 1.51 ± 0.14 (p < 0.05) ). At the end of storage, the tissue level of myeloperoxidase (Units/g wet tissue) was significantly lower for Group C than for Groups A and D (C vs. A or B or D:13.5± 18.4 vs. 42.2± 14.2 (p <0.05) or 40.8± 52.2 (N.S.) or 42.6± 18.8 (p < 0.05)) . In conclusion, the PAF antagonist, TCV-309 , is effective for the prevention of ischemic reperfusion injury in 24-hour cold preserved canine lungs.
URI: http://hdl.handle.net/10069/15982
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 39, No. 1-3

Citable URI : http://hdl.handle.net/10069/15982

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