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Efficancy of a Rinse Solution for Prevention of Reperfusion Injury in Canine 24-hour Cold Preserved Lungs

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Title: Efficancy of a Rinse Solution for Prevention of Reperfusion Injury in Canine 24-hour Cold Preserved Lungs
Authors: Shingu, Hiroshi
Issue Date: 25-Oct-1994
Citation: Acta medica Nagasakiensia. 1994, 39(1-3), p.119-125
Abstract: The efficacy of a rinse solution for prevention of ischemiareperfusion injury in the lung preserved for 24 hours was evaluated by using the canine isolated lung perfusion model. The heart and the lungs were harvested and preserved for 24 hours at 4-6℃ in modified:Euro-Collins(EC)solution. Prostaglandin El (100 p g) was adiministered into the pulmonary trunk prior to flushing canine lungs with preservation solution. The next day, left lungs were isolated with a perfluorochemical (FC-43) or Carolina rinse II solution. Immediately after rinsing, the lung was reperfused with homologous venous blood (400 ml) for 120 min. in the experimental pump circulation system. The lungs were classified into the following six groups: Group 1 (n = 5), without rinsing (control group), Group 2 (n = 6) , rinsing with oxygenated FC-43 solution (250 ml), Group 3 (n = 4), rinsing with non-oxygenated FC-43 solution (250 ml), Group 4 (n = 5), rinsing with pluronic F 68 solution (250 ml), Group 5 (n = 4), rinsing with the Carolina Rinse Solution a (250 ml) and Group 6 (n = 4). rinsing with the Carolina Rinse Solution 11 (250 ml) in addition with hydroxyethyl starch (50 g/l). Pulmonary vascular resistance and lung water volume (wet-dry/wet ratio) were lower and dynamic lung compliance was higher in Group 2 than in other groups. The tissue myeloperioxidase level in Group 2 was significantly lower than those in Groups 4, 5 and 6 (p < 0.05). Histologically, severe marked lung edema was observed in Groups 1, 5 and 6. The lung showed almost normal architecture in Group 2. In conclusion, terminal rinsing with oxygenated FC-43 solution immediatly before blood reperfusion is useful for prevention of ischemia-reperfusion injury after 24-hour cold ischemic storage in modified Euro-Collins solution.
URI: http://hdl.handle.net/10069/15983
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 39, No. 1-3

Citable URI : http://hdl.handle.net/10069/15983

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