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Recombination Activating Gene (RAG)-1 and 2 Encoding Proteins Expressed by the Baculovirus System


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Title: Recombination Activating Gene (RAG)-1 and 2 Encoding Proteins Expressed by the Baculovirus System
Authors: Araki, Ryoko / Kaku, Mitsuo / Itoh, Masahiro / Hamatani, Kiyohiro / Usui, Toshiaki / Abe, Masumi
Issue Date: 20-Jun-1996
Citation: Acta medica Nagasakiensia. 1996, 41(1-2), p.43-47
Abstract: We have been attempting to obtain mouse recombination activating gene-1 (RAG-1) and RAG-2 protein for biochemical analyses. First of all, we obtained truncated products of these genes expressed and purified using the E. coli expression system and then established the polyclonal and monoclonal antibodies by means of E. coli expressed peptides as antigens. Subsequently, whole RAG-1 and RAG-2 gene products were expressed the baculovirus expression system. Since it has been difficult to achieve the significant gene expression of full-lenght cDNA, we employed the glutathione S-transferase (GST)-fused gene-expression system which facilitated the massive expression of gene products. This system was also advantageous in that we could detect the expressed protein molecules not only with anti-RAG antibody but also with anti-GST antibody.
URI: http://hdl.handle.net/10069/16049
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 41, No. 1-2

Citable URI : http://hdl.handle.net/10069/16049

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