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Amino Acid Sequence of Short Subfragment-2 from Adult Chicken Gizzard Muscle Myosin


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タイトル: Amino Acid Sequence of Short Subfragment-2 from Adult Chicken Gizzard Muscle Myosin
著者: Watanabe, Bunji / Tanigawa, Mihoko
発行日: 1996年 6月20日
引用: Acta medica Nagasakiensia. 1996, 41(1-2), p.58-66
抄録: The complete amino acid sequence of the short subfragment- 2 (short S-2) from adult chicken gizzard muscle has been determined by direct analysis of peptides derived from digests of this portion with cyanogen bromide, lysyl endopeptidase and from hydrolysates of CNBr fragments with dilute formic acid. It contains 323 amino acid residues which span the NH2-terminus of the subfragment-2 (S-2) to the NH2 -terminal portion of the hinge region in S-2. Although, structural differences between embryonic and adult chicken gizzard muscle myosin heavy chain (MHC) have been suggested, comparison of the amino acid sequence of the short S-2 from adult chicken gizzard muscle MHC with that from embryonic chicken gizzard MHC, deduced from its nucleotide sequence of the cDNA, showed that the sequence were identical. The amino acid sequence of the short S-2 from adult chicken gizzard muscle MHC was compared with the corresponding sequence of chicken brain myosin, Drosophila non-muscle myosin, chicken skeletal muscle myosin and chicken cardiac ventricular muscle myosin. The results show relatively higher sequence identities with chicken brain myosin (76.5%) and Drosophila non-muscle myosin (60.0%) and lower identities with chicken skeletal muscle myosin (36.8%) and chicken cardiac ventricular muscle myosin (36.5%).
キーワード: amino acid sequence / smooth muscle myosin / myosin short subfragment-2.
URI: http://hdl.handle.net/10069/16052
ISSN: 00016055
資料タイプ: Departmental Bulletin Paper
原稿種類: publisher
出現コレクション:Volume 41, No. 1-2

引用URI : http://hdl.handle.net/10069/16052

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