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In Situ Hybridization Techniques for Electron Microscopy


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Title: In Situ Hybridization Techniques for Electron Microscopy
Authors: Izumi, Shin-Ichi / Nakane, Paulk.
Issue Date: Dec-1996
Citation: Acta medica Nagasakiensia. 1996, 41(3-4), p.1-7
Abstract: In order for messenger RNA (mRNA) to be localized in cells and tissues by in situ hybridization at the electron microscopic level, the following conditions must be accomplished. The target mRNA must be retained in the specimen with well-preserved morphology. The labeled-nucleic acid probes must be accessible to the mRNA and make hybrids with the mRNA efficiently. The labels should be detectable by either transmission or scanning electron microscopes. To achieve these, appropriate techniques for the electron microscopic in situ hybridization (EMISH) for the target mRNA were developed and have been improved. This review will describe representative procedures of EMISH of mRNA and will make some comments on the procedures.
Keywords: in situ hybridization / electron microscopy / backscattered electron imaging / RNA
URI: http://hdl.handle.net/10069/16057
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 41, No. 3-4

Citable URI : http://hdl.handle.net/10069/16057

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