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Mapping and Ordering Cosmid Clones Obtained from Human Chromosome Region 11 q 13.4-q25 by Fluorescence in situ Hybridization


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タイトル: Mapping and Ordering Cosmid Clones Obtained from Human Chromosome Region 11 q 13.4-q25 by Fluorescence in situ Hybridization
著者: Kato, Rumiko
発行日: 1996年12月
引用: Acta medica Nagasakiensia. 1996, 41(3-4), p.26-30
抄録: A cosmid library was screened with 40 sequence-tagged sites (STSs) designed previously on the basis of DNA sequences of microclones that were generated by chromosome microdissection on two different human chromosomal regions, 11q14-q22 and 11q23-q25. Seventeen cosmid clones that corresponded to the STSs were successfully isolated. Single-color fluorescence in situ hybridization (FISH) using the 17 cosmids as probes, 11 were mapped to the expected regions of 11q. Then, the order of localization of the 7 cosmids (cG20, cG38, cG38R, cG50, cG51, cG75 and cR54) at the 11q21-q22.3 region were determined by two-color FISH on the extended prometaphase chromosomes, as cen-cG50-cG75- cG51-cG38-cG38R-cG20-cR54-tel. The result not only confirmed that microdissection is useful in the isolation of region-specific DNA clones, but also indicated that subsequent single- or two-color FISH is a valuable tool to map novel clones and marker DNAs, especially their order in the human genome.
URI: http://hdl.handle.net/10069/16061
ISSN: 00016055
資料タイプ: Departmental Bulletin Paper
原稿種類: publisher
出現コレクション:Volume 41, No. 3-4

引用URI : http://hdl.handle.net/10069/16061

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