DSpace university logo mark
Advanced Search
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > School of Medicine > Bulletin > Acta Medica Nagasakiensia > Volume 41, No. 3-4 >

Mapping and Ordering Cosmid Clones Obtained from Human Chromosome Region 11 q 13.4-q25 by Fluorescence in situ Hybridization


File Description SizeFormat
acta41_03_05_t.pdf780.4 kBAdobe PDFView/Open

Title: Mapping and Ordering Cosmid Clones Obtained from Human Chromosome Region 11 q 13.4-q25 by Fluorescence in situ Hybridization
Authors: Kato, Rumiko
Issue Date: Dec-1996
Citation: Acta medica Nagasakiensia. 1996, 41(3-4), p.26-30
Abstract: A cosmid library was screened with 40 sequence-tagged sites (STSs) designed previously on the basis of DNA sequences of microclones that were generated by chromosome microdissection on two different human chromosomal regions, 11q14-q22 and 11q23-q25. Seventeen cosmid clones that corresponded to the STSs were successfully isolated. Single-color fluorescence in situ hybridization (FISH) using the 17 cosmids as probes, 11 were mapped to the expected regions of 11q. Then, the order of localization of the 7 cosmids (cG20, cG38, cG38R, cG50, cG51, cG75 and cR54) at the 11q21-q22.3 region were determined by two-color FISH on the extended prometaphase chromosomes, as cen-cG50-cG75- cG51-cG38-cG38R-cG20-cR54-tel. The result not only confirmed that microdissection is useful in the isolation of region-specific DNA clones, but also indicated that subsequent single- or two-color FISH is a valuable tool to map novel clones and marker DNAs, especially their order in the human genome.
URI: http://hdl.handle.net/10069/16061
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 41, No. 3-4

Citable URI : http://hdl.handle.net/10069/16061

All items in NAOSITE are protected by copyright, with all rights reserved.

 

Valid XHTML 1.0! Copyright © 2006-2015 Nagasaki University Library - Feedback Powerd by DSpace