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A 1.5-Mb PAC/BAC Contig Spanning the Prader-Willi Syndrome Critical Region (PWCR)

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Title: A 1.5-Mb PAC/BAC Contig Spanning the Prader-Willi Syndrome Critical Region (PWCR)
Authors: Kondo, Shinji / Tomita, Hiroaki / Kishino, Tatsuya / Yoshiura, Kohichiro / Yamada, Koki / Soeda, Eiichi / Matsumoto, Naomichi / Ohta, Tohru / Fujii, Tohru / Niikawa, Norio
Issue Date: 14-Jun-2000
Citation: Acta medica Nagasakiensia. 2000, 45(1-2), p.43-46
Abstract: Prader-Willi syndrome (PWS) is a multiple anomalies/mental retardation syndrome. The putative PWS gene(s) remains unknown, and its occurrence is based on genomic imprinting at chromosome 15q11-q13. We have constructed a 1.5- Mb, fine, physical map of PWS critical region (PWCR) between two markers, D15S9 and D15S174 at 15q11-q13. The map is composed of 32 PAC and 3 BAC clones without any gaps. By the PAC/BAC-end sequencing procedure, a total of 26 sequence tag site (STS) markers were newly generated, and 5 expressed sequence tags (ESTs) were mapped in the region. The contig map was verified by both STS and fluorescence in situ hybridization analyses. Our map has higher resolution, compared with a previous YAC-based map of PWCR. It is useful for further genome analysis, especially on genomic imprinting of this region.
Keywords: Prader-Willi syndrome critical region (PWCR) / 15q 11-q 13 / imprinted region / PAC/BAC contig;physical map
URI: http://hdl.handle.net/10069/16157
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 45, No. 1-2

Citable URI : http://hdl.handle.net/10069/16157

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