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Clinical Significance of Telomerase Activity and Telomere Length in Various Liver Diseases


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Title: Clinical Significance of Telomerase Activity and Telomere Length in Various Liver Diseases
Authors: Kitaoka, Fumio / Takayama, Kazuyuki / Okudaira, Sadayuki / Fujioka, Hikaru / Furui, Junichiro / Kanematsu, Takashi
Issue Date: 25-Jun-2003
Citation: Acta medica Nagasakiensia. 2003, 48(1-2), p.55-60
Abstract: We investigated the clinical significance of telomerase activity and telomere length in hepatocellular carcinomas (HCCs) and chronic liver diseases. Telomerase activity was assessed quantitatively using 'Stretch PCR' assay and telomere length by Southern blotting in 24 HCCs, 2 focal nodular hyperplasia, 8 liver cirrhosis, 10 chronic hepatitis and 8 histologically normal livers. The latter were obtained from normal sections of resected specimens of cholangiocellular carcinoma, metastatic liver tumor or hemangioma. The relative titers of telomerase activity (RTA) were significantly higher in HCCs (average, 54 units) than in chronic liver diseases (average, 0.6 units) (P<0.001). In comparison, RTA was less than 2 units in non-malignant liver tissues. Telomere length in cirrhotic liver tissues was significantly shorter than in normal livers and tended to be shorter than those in chronic hepatitis. Telomere length and RTA correlated with the grading of tissue derangement in chronic liver diseases. Our results suggest that RTA estimated by Stretch PCR assay might be clinically useful for accurate diagnosis of liver diseases, particularly HCCs. In addition, telomere length seems to having a possibility as a useful predictor for risk of hepatocarcinogenesis.
Keywords: telomerase activity / telomere length / hepatocellular carcinoma / chronic liver disease / Stretch PCR
URI: http://hdl.handle.net/10069/16243
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 48, No. 1-2

Citable URI : http://hdl.handle.net/10069/16243

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