DSpace university logo mark
Advanced Search
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > School of Medicine > Bulletin > Acta Medica Nagasakiensia > Volume 52, No. 4 >

Protective Immunity Against Challenge Infection with Trichinella spiralis in the Rat


File Description SizeFormat
acta_52_4_101.pdf4.11 MBAdobe PDFView/Open

Title: Protective Immunity Against Challenge Infection with Trichinella spiralis in the Rat
Authors: Quan, Fu Shi / Bae, Jun Sang / Lee, Jeong Beom / Min, Young Ki / Yang, Hun Mo / Joo, Kyung-Whan / Cho, Sung-Weon / Lee, Joon-Sang / Nakayama, Toshiyuki / Sekine, Ichiro
Issue Date: Mar-2008
Citation: Acta medica Nagasakiensia. 2007, 52(4), p.101-106
Abstract: Protective immunity and immune response were studied in rats after primarily infection with Trichinella spiralis. The parameters measured include serum antibody isotype responses, mesenteric lymph node (MLN) cell and spleen cell proliferation responses in vitro, and the levels of interferon (IFN)-γ, interleukin (IL)-2, IL-4 and IL-10 as markers of the T-helper (Th) subset. Protective immunity was assessed by the degree of expulsion of adult worms from the rat intestine. Protective immunity against adult worms after challenge infection was 99.80%. Although IFN-γ, IL-2, IL-4 and IL-10 in both the MLNs and spleen were detected, IFN-γ and IL-2 levels were higher in the spleen than in the MLNs, and IL-4 and an increased amount of IL-10 was released in the MLNs as compared to the spleen. The levels of the specific immunoglobulins (Ig) G, IgM, IgG1 and IgG2a on week 6 after primary infection, and on day 7 after challenge infection, were higher as compared to levels in uninfected rats that only received a challenge infection (P ? 0.001), whereas the antibody level of IgG1 was significantly elevated from that of IgG2a (P ? 0.001). These results demonstrate that Th1 type responses predominated. In addition, as Th2 type cytokines were also produced, it is proposed that the protective immunity by primary infection was also related to the Th1 type responses.
Keywords: Trichinella spiralis / Infection / Excretory-secretory antigen / Antibody isotype response / T helper type cytokine
URI: http://hdl.handle.net/10069/16824
ISSN: 00016055
Relational Links: http://joi.jlc.jst.go.jp/JST.JSTAGE/amn/52.101
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 52, No. 4

Citable URI : http://hdl.handle.net/10069/16824

All items in NAOSITE are protected by copyright, with all rights reserved.

 

Valid XHTML 1.0! Copyright © 2006-2015 Nagasaki University Library - Feedback Powerd by DSpace