DSpace university logo mark
Advanced Search
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > School of Medicine > Bulletin > Acta Medica Nagasakiensia > Volume 52, No. 4 >

Hyperosmolarity Attenuates the Contraction of Rat Trachea Through the Inhibition of Phosphatidylinositol Response


File Description SizeFormat
acta_52_4_107.pdf3.85 MBAdobe PDFView/Open

Title: Hyperosmolarity Attenuates the Contraction of Rat Trachea Through the Inhibition of Phosphatidylinositol Response
Authors: Saito, Masataka / Shibata, Osamu / Matsunaga, Shoji / Tsuda, Atsushi / Makita, Tetsuji / Sumikawa, Koji
Issue Date: Mar-2008
Citation: Acta medica Nagasakiensia. 2007, 52(4), p.107-110
Abstract: Although hyperosmolarity associated with diabetes is known to attenuate contractile response of airway smooth muscle, intracellular mechanisms involved are not fully understood. We examined the effects of hyperosmolarity on carbachol (CCh)- and aluminum fluoride (AF)-induced contractile and phosphatidylinositol (PI) responses of rat trachea. In vitro measurements of isometric tension and [3H] inositol monophosphate (IP1) formed were conducted by using rat tracheal rings and slices. Hyperosmolarity solutions of 350, 450 and 600 mOsm were made with dissolving glucose in Krebs-Henseleit (K-H) solution. Hyperosmolarity attenuated dose-dependently CCh-induced contraction of rat trachea (1.86 ± 0.13 g at 300 mOsm, 1.85 ± 0.16 g at 350 mOsm, 1.37 ± 0.07 g at 450 mOsm and 0.50 ± 0.04 g at 600 mOsm, respectively), and also attenuated CCh- induced IP1 accumulation (5.77 ± 0.33 Bq at 300 mOsm, 3.38 ± 0.26 Bq at 350 mOsm, 2.08 ± 0.30 Bq at 450 mOsm and 1.71 ± 0.40 Bq at 600 mOsm, respectively), and AF-induced IP1 accumulation (3.93 ± 0.22 Bq at 300 mOsm, 1.63 ± 0.14 Bq at 450 mOsm and 1.02 ± 0.14 Bq at 600 mOsm, respectively). The results suggest that hyperosmolarity would inhibit G-protein-coupled phospholipase C, resulting in attenuation of CCh-induced airway smooth muscle contraction.
Keywords: Hyperosmolarity / Contractile and phosphatidylinositol (PI) responses / Rat trachea
URI: http://hdl.handle.net/10069/16825
ISSN: 00016055
Relational Links: http://joi.jlc.jst.go.jp/JST.JSTAGE/amn/52.107
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 52, No. 4

Citable URI : http://hdl.handle.net/10069/16825

All items in NAOSITE are protected by copyright, with all rights reserved.

 

Valid XHTML 1.0! Copyright © 2006-2015 Nagasaki University Library - Feedback Powerd by DSpace