DSpace university logo mark
詳細検索
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > 030 医学部 > 030 紀要 > Acta Medica Nagasakiensia > Volume 37, No. 1-4 >

Analysis of Numerical Chromosome Aberration of Gastric Cancer : Application of Fluorescent in situ Hybridization using Chromosome Specific DNA Probes.


ファイル 記述 サイズフォーマット
acta37_01-04_37_t.pdf2.87 MBAdobe PDF本文ファイル

タイトル: Analysis of Numerical Chromosome Aberration of Gastric Cancer : Application of Fluorescent in situ Hybridization using Chromosome Specific DNA Probes.
著者: Hiroyuki, Yamaguchi
発行日: 1992年12月25日
引用: Acta Medica Nagasakiensia. 1992, 37(1-4), p.163-170
抄録: An analysis in the numerical chromosome aberration of human gastric cancer was made by applying fluorescent in situ hybridization (FISH) with chromosome specific DNA probes. Thirteen primary tumors and four metastatic lymph nodes were surgically resected from thirteen gastric cancer patients and analyzed. FISH using α satellite DNA probes of chromosomes 1, 3, 7, 11, 17,and X was applied to interphase cells of each sample, then signal spot number in each nucleus was counted. DNA ploidy from the flow cytometric analysis of nuclear DNA content was compared with chromosomal aberration from FISH analysis. Numerical chromosome aberration, especially a gain in chromosomes, occurred more frequently in DNA aneuploid cancers than in DNA diploid cancers. Numerical gain of chrmosomes 7 and 17 were significantly more frequent it DNA aneuploid cancers. Since gastric cancer with a gain in chromosome 7 and/or 17 is often accompanied with a high level of metastasis in lymph nodes and/or distant metastasis, it is suggested that numerical gain of those chromosomes must be related to the abillity of metastasis or growth in metastatic regions, Since the FISH study could detect numerical chromosome aberration in DNA diploid cancer, we will be able to further study the DNA diploidy of clinical materials by using this technique. Because FISH has made it possible to detect the chromosome number rapidly in the interphase cells, it is hopeful that human solid cancer chromosome analysis will rise as these materials for analysis increase and that we will take long strides in cytogenetic research of cancer in the near future.
URI: http://hdl.handle.net/10069/17582
ISSN: 00016055
資料タイプ: Departmental Bulletin Paper
原稿種類: publisher
出現コレクション:Volume 37, No. 1-4

引用URI : http://hdl.handle.net/10069/17582

このリポジトリに保管されている文献はすべて著作権により保護されています。
印刷やダウンロード等データの複製は、調査研究・教育または学習を目的とする場合に限定されます。

 

Valid XHTML 1.0! Copyright © 2006-2015 長崎大学附属図書館 - お問い合わせ Powerd by DSpace