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A Study of the sequential demonstration of nucleolar organizer regions and PCNA immnolabelling in colorectal carcinomas


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Title: A Study of the sequential demonstration of nucleolar organizer regions and PCNA immnolabelling in colorectal carcinomas
Authors: Yoshida, Kazuya / Tomita, Masao
Issue Date: 14-Jan-1993
Citation: Acta Medica Nagasakiensia. 1993, 38(1), p.15-20
Abstract: The possibility of a sequential demonstration of the nucleolar organizer regions (NORs) and proliferating cell nuclear antigen (PCNA) by the argyrophil (AgNOR) method and immunostaining, respectively, was explored in colorectal carcinomas. Both PCNA and the argyrophilic proteins of NORs (AgNORs) were clearly visualized when immunostaining of PCNA by the labelled streptavidine-biotin (LSAB) method was performed before the AgNOR method. A comparative study revealed that the PCNA labelling index determined with sections stained for PCNA alone was 1.8 to 6% greater than that determined by the sequential double staining method. The difference in AgNOR number per nucleus was not statistically significant, suggesting that the double staining method can be used for determining both the AgNOR number and PCNA labelling index. This double-staining method was used to study AgNORs and PCNA in 12 patients with colorectal cancer. The mean AgNOR numbers for PCNA positive and negative cells were 11.9 and 3.4, respectively. The former number was significantly greater than the later (p < 0.0001), indicating that the interphase AgNOR number determined by the double-staining method reflects the proliferating activity of the cells. Separate enumeration of NORs by this method in dividing and nondividing cancer cells is expected to provide important information for the analysis of cancer cell kinetics. In this study, the validity of double stainig of PCNA and AgNOR was defined by the result that more than 10 of AgNOR counts in positive PCNA cells of double staining method indicate poor prognosis, on the contrary, less than 4 of AgNOR counts in negative PCNA cells imply fair prognosis in patients with Dukes C of colorectal cancer.
URI: http://hdl.handle.net/10069/17597
ISSN: 00016055
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:Volume 38, No. 1

Citable URI : http://hdl.handle.net/10069/17597

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