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Analysis of Mutations in the Human HPRT Gene Induced by Accelerated Heavy-Ion Irradiation


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Title: Analysis of Mutations in the Human HPRT Gene Induced by Accelerated Heavy-Ion Irradiation
Authors: Kagawa, Yasuhiro / Yatagai, Fumio / Suzuki, Masao / Kase, Youko / Kobayashi, Akiko / Hirano, Masahiko / Kato, Takesi / Watanabe, Masami / Hanaoka, Fumio
Authors (alternative): 谷田貝, 文夫
Issue Date: Sep-1995
Publisher: 日本放射線影響学会
Citation: Journal of radiation research. 1995, 36(3), p.185-195
Abstract: Multiplex PCR analysis of HPRT(-) mutations in human embryo (HE) cells induced by 230 keV/μm carbon-ion irradiation showed no large deletion around the exon regions of the locus gene in contrast to the irradiations at different LETs. To identify these mutations, the sequence alterations in a cDNA of hprt gene were determined for 18 mutant clones in this study. Missing of exon 6 was the most frequent mutational event (10 clones), and missing of both exons 6 and 8 was next most frequent event (6 clones), then base substitutions (2 clones). These characteristics were not seen in a similar analysis of spontaneous mutations, which showed base substitution (5 clones), frameshift (2 clones), missing of both exons 2 and 3 (2 clones), and a single unidentified clone. Direct sequencing and restriction enzyme digestion of the genomic DNA of the mutants which showed missing of exons 6 and 8 in the cDNA, supports the possibility that they were induced by aberrant mRNA splicing.
Keywords: Mutation / HPRT / Heavy-Ion Irradiation / Aberrant mRNA Splicing / High LET
URI: http://hdl.handle.net/10069/18734
ISSN: 04493060
Relational Links: http://ci.nii.ac.jp/naid/110002328080/
Rights: 日本放射線影響学会 / 本文データは学協会の許諾に基づきCiNiiから複製したものである
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/18734

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