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The antioxidative activity of Kangen-karyu extract delays senescence of human lung fibroblasts


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Title: The antioxidative activity of Kangen-karyu extract delays senescence of human lung fibroblasts
Authors: Satoh, Akiko / Yokozawa, Takako / Tanaka, Takashi / Okamoto, Takuya / Sei, Yasuo
Authors (alternative): 横澤, 隆子
Issue Date: 20-Apr-2004
Publisher: 和漢医薬学会
Citation: 和漢医薬学雑誌. 2004, 21(2), p.87-93
Abstract: Replicative senescence (RS) of human diploid fibro blasts (HDFs) has become a classical model of aging and HDFs, such as WI-38 cells, display increased cellular oxidant production associated with RS. Several phenomena associated with RS are also observed in stress-induced replicative senescence (SIPS). In particular, SIPS of WI-38 cells caused by hydrogen peroxide (H_2O_2) is a useful and reasonable cellular aging model for evaluating the effects of potential anti-aging agents against oxidative stress. We used this well-established model to evaluate the anti-aging effect of Kangen-karyu, focusing on its antioxidant activity. Treatment of WI-38 cells undergoing SIPS caused by H_20_2 with Kangen-karyu extract significantly reduced reactive oxygen species (ROS) generation and lipid peroxidation levels. In addition, the intracellular GSH levels, reflecting cellular ROS generation, were reduced by treatment with Kangen-karyu extract. These results suggest that Kangen-karyu attenuated the age-associated increase of cellular oxidative damage. Moreover, Kangen-karyu extract normalized the G_0/G_1 phase arrest and reversed the diminished cell viability resulting from exposure to H_2O_2. Furthermore, the extract prolonged the lifespan of WI-38 cells undergoing SIPS. This study suggests that Kangen-karyu may delay the aging process in cells undergoing SIPS by attenuating oxidative damage.
Keywords: Kangen-karyu / aging / WI-38 / hydrogen peroxide / free radical
URI: http://hdl.handle.net/10069/18745
ISSN: 18801447
Relational Links: http://ci.nii.ac.jp/naid/110001846625/
Rights: 和漢医薬学会 / 本文データは学協会の許諾に基づきCiNiiから複製したものである
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/18745

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