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ミニブタの同種末梢血リンパ球前処置による in vitro IL-2様活性の早期検出


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Title: ミニブタの同種末梢血リンパ球前処置による in vitro IL-2様活性の早期検出
Other Titles: Early In Vitro Detection of Interleukin-2-Like Activities by Pretreatment with Allogeneic Blood Lymphocytes in Miniature Swine
Authors: 田浦, 保穂 / 角田, 司 / 塩竃, 利昭 / Mullen, Yoko / Papoian, Tom
Authors (alternative): Taura, Yasuho / Tsunoda, Tsukasa / Shiogama, Toshiaki
Issue Date: 15-Jun-1991
Publisher: 社団法人日本獣医学会
Citation: The journal of veterinary medical science. 1991, 53(3), p.365-369
Abstract: ミニブタの同種移植における免疫反応の指標を得る目的で, ドナー末梢血リンパ球(PBL)前処置した後, ドナーPBLに対する in vitroでのIL-2様活性の測定を行い, 以下の結果が得られた: (1)MHC不適合無処置群の48時間MLC上清中にIL-2様活性がみられ, 48時間培養の方が高値であった; (2)MHC不適合PBLを1回移植した群は, 無処置群よりも in vitroでのIL-2様活性は高値であった; (3)MHC不適合PBLを週1回計4回移植した群は, in vitroでのIL-2様活性の検出がやや早期に認められた. / In order to determine the means of monitoring the immunological status of allograft recipients in miniature swine, an assay was developed to measure interleukin (IL)-2 production in vitro by pretreatment of donor peripheral blood lymphocytes (PBL). Miniature swine were given 0 to 4 weekly intravenous transfusions of 5-10×10^7 donor PBL incompatible at major histocompatibility complex (MHC) and assayed in vitro for donor specific immune IL-2-like activities. The results are summarized as follows: (1) IL-2-like activity in 24 hr and 48 hr supernatants from mixed lymphocyte cultures (MLC) with MHC-incompatible PBL was detected without pretreatment. The 48 hr MLC supernatant exhibited a high IL-2-like activity compared with the 24 hr; (2) IL-2-like activity after only one transfusion with MHC-incompatible PBL was higher than that without pretreatment; (3) IL-2-like activity in 4 weekly transfusions was detectable slightly earlier than that without pretreatment or three transfusions with MHC-incompatibile PBL.
Keywords: IL-2-like activity / in vitro assay / miniature swine / monitor
URI: http://hdl.handle.net/10069/18946
ISSN: 09167250
Relational Links: http://ci.nii.ac.jp/naid/110003947860/
Rights: 社団法人日本獣医学会 / 本文データは学協会の許諾に基づきCiNiiから複製したものである
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/18946

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