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The Protective Effect of Fermented Milk Kefir on Radiation-induced Apoptosis in Colonic Crypt Cells of Rats The Protective Effect of Fermented Milk Kefir on Radiation-induced Apoptosis in Colonic Crypt Cells of Rats


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Title: The Protective Effect of Fermented Milk Kefir on Radiation-induced Apoptosis in Colonic Crypt Cells of Rats The Protective Effect of Fermented Milk Kefir on Radiation-induced Apoptosis in Colonic Crypt Cells of Rats
Authors: Matsuu, Mutsumi / Shichijo, Kazuko / Okaichi, Kumio / Wen, Chun Yang / Fukuda, Eiichiro / Nakashima, Masahiro / Nakayama, Toshiyuki / Shirahata, Sanetaka / Tokumaru, Sennosuke / Sekine, Ichiro
Issue Date: Jun-2003
Publisher: 日本放射線影響学会
Citation: Journal of radiation research. 2003, 44(2), p.111-115
Abstract: To evaluate the effect of fermented milk kefir on X-ray-induced apoptosis in the colon of rats, we examined the apoptotic index, the mean number of apoptotic cells detected by H&E staining per crypt in the colon, in control rats and kefir-pretreated rats drinking kefir for 12 days before irradiation. Apoptotic cells were confirmed by TUNEL staining, and active caspase-3 expression was studied by immunohistochemistry. The cell position of apoptotic cells and active caspase-3 positive cells were examined. The apoptotic index of kefirtreated rats was significantly (p < 0.05) decreased 2 h after 1 Gy irradiation in comparison with control rats at crypt cell positions 1-3, 5-7, 13, and 15. Active caspase-3 expression in the kefir-treated rats was also significantly (p < 0.05) reduced in comparison with control rats 2 h after 1 Gy irradiation at crypt cell positions 1-4, 13, and 15. This study indicated that kefir protects colonic crypt cells against radiation-induced apoptosis, which was most pronounced in the stem cell region of the crypt. The antiapoptotic effect of fermented milk kefir was due to the inhibition of caspase-3 activation.
Keywords: Kefir / Radiation-induced apoptosis / X-ray / Rats / Colon
URI: http://hdl.handle.net/10069/19110
ISSN: 04493060
Relational Links: http://ci.nii.ac.jp/naid/110002328873/
Rights: 日本放射線影響学会 / 本文データは学協会の許諾に基づきCiNiiから複製したものである
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/19110

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