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Plectasin has antibacterial activity and no affect on cell viability or IL-8 production

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Title: Plectasin has antibacterial activity and no affect on cell viability or IL-8 production
Authors: Hara, Shintaro / Mukae, Hiroshi / Sakamoto, Noriho / Ishimoto, Hiroshi / Amenomori, Misato / Fujita, Hanako / Ishimatsu, Yuji / Yanagihara, Katsunori / Kohno, Shigeru
Issue Date: 3-Oct-2008
Publisher: Elsevier
Citation: Biochemical and Biophysical Research Communications, 374(4), pp.709-713; 2008
Abstract: Animals and plants express endogenous peptide antibiotics called defensins. Defensins show broad-spectrum antimicrobial activity, even against bacteria that have resistance to conventional antibiotics, which has made them viable candidates for new antibiotics. However, human defensins have failed to reach the market because of their cytotoxic effects and non-antimicrobial bioactivities. Plectasin is a defensin that has shown promise but has not had its potentially negative effects clarified. To address this issue, we examined plectasin’s cytotoxicity in human cells using an AlamarBlue reduction assay, its interleukin (IL)-8-inducing capacity using real-time PCR and ELISA, and measured its MIC against bacteria. We confirmed that plectasin has specific antibacterial activity against Streptococcus pneumoniae. Plectasin showed no cytotoxicity to A549 cells, normal human bronchial epithelial cells, or lung fibroblasts, and it did not induce IL-8 transcription or production in A549 cells. Our results suggest that plectasin could be an inoffensive alternative antibiotic for clinical application.
Keywords: Plectasin / Human defensins / Antibacterial activity / Cytokine induction / Cytotoxicity / Defensin / Human neutrophil peptide-1 / Interleukin-8 induction / A549 cell / Normal human bronchial epithelial cell / Normal human lung fibroblast
URI: http://hdl.handle.net/10069/19201
ISSN: 0006291X
DOI: 10.1016/j.bbrc.2008.07.093
PubMed ID: 18675251
Rights: Copyright (c) 2008 Elsevier Inc. All rights reserved.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/19201

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