DSpace university logo mark
Advanced Search
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > University Hospital > Articles in academic journal >

Influence of murine hepatitis induced by D-(+)-galactosamine hydrochloride and lipopolysaccharide on gene expression of polyethylenimine/plasmid DNA polyplex


File Description SizeFormat
BPB31_1585.pdf800.03 kBAdobe PDFView/Open

Title: Influence of murine hepatitis induced by D-(+)-galactosamine hydrochloride and lipopolysaccharide on gene expression of polyethylenimine/plasmid DNA polyplex
Authors: Miyanaga, Kei / Yoshioka, Takashi / Nakagawa, Hiroo / Kitahara, Takashi / To, Hideto / Ichikawa, Nobuhiro / Nakashima, Mikiro / Nishida, Koyo / Nakamura, Junzo / Sasaki, Hitoshi
Issue Date: Aug-2008
Publisher: Pharmaceutical Society of Japan
Citation: Biological and Pharmaceutical Bulletin, 31(8), pp.1585-1589; 2008
Abstract: We investigated the influence of murine hepatitis induced by D-(+)-galactosamine and lipopolysaccharide (DGalN/LPS) on polyethylenimine (PEI)-mediated plasmid DNA (pDNA) delivery. pDNA encoding firefly luciferase was used as the model reporter gene. PEI was used as the non-viral vector because of its high gene expression and low toxicity. The activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in mice indicated the highest peaks at 12 h after D-GalN/LPS injection, then the activities of serum ALT and AST rapidly decreased. We determined luciferase activity in various organs of D-GalN/LPS-treated mice and control mice after an intravenous administration of PEI/pDNA complexes. High transgene expression was observed in the liver, spleen, and lung of both mice. Compared to the control mice, a significant increase of transgene expression was observed in the liver of D-GalN/LPS-treated mice after D-GalN/LPS injection. The transgene expression in the spleen and lung decreased at 6 and 12 h after D-GalN/LPS injection. In conclusion, we found that murine hepatitis induced by D-GalN/LPS injection can influence PEI-mediated pDNA delivery and its influence was different from that induced by CCl4 injection which was reported previously. These results demonstrated the necessity of considering the timing and dose of gene therapy according to the disease and its stage.
Keywords: Disease stage / Gene delivery / Murine hepatitis / Non-viral vector / Polyethylenimine
URI: http://hdl.handle.net/10069/19219
ISSN: 09186158
DOI: 10.1248/bpb.31.1585
PubMed ID: 18670093
Rights: (c) 2008 The Pharmaceutical Society of Japan
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/19219

All items in NAOSITE are protected by copyright, with all rights reserved.

 

Valid XHTML 1.0! Copyright © 2006-2015 Nagasaki University Library - Feedback Powerd by DSpace