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Morphologic and Molecular Characterization of Isospora belli Oocysts from Patients in Thailand

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タイトル: Morphologic and Molecular Characterization of Isospora belli Oocysts from Patients in Thailand
著者: Jongwutiwes, Somchai / Putaporntip, Chaturong / Charoenkorn, Malee / Iwasaki, Takuya / Endo, Takuro
発行日: 2007年 7月
出版者: American Society of Tropical Medicine and Hygiene
引用: American Journal of Tropical Medicine and Hygiene, 77(1), pp.107-112; 2007
抄録: To investigate the extent of genetic heterogeneity in the genus Isospora infecting patients in Thailand, a total of 38 fecal samples containing Isospora oocysts from human immunodeficiency virus/acquired immunodeficiency syndrome patients (n = 30), corticosteroid-treated patients (n = 3) and immunocompetent individuals (n = 5) were recruited for analysis. Remarkable variation in the maximum width and length of oocysts both within and between isolates was observed. However, the average length-width ratio of oocysts was within the range for I. belli (> 1.2). Ex vivo sporogonic development of freshly passed oocysts in feces from three of these isolates was observed longitudinally, showing that 27% of these oocysts underwent complete sporulation. Interestingly, 95% of sporulated oocysts contained two sporocysts in an oocyst with four sporozoites in each sporocyst, and Caryospora-like oocysts, characterized by eight sporozoites enclosed by a single sporocyst, were also detected (5%). The small subunit ribosomal RNA (rRNA), 5.8S rRNA, internal transcribed spacer 1 (ITS-1), and ITS-2 were highly conserved, indicating that there were no cryptic species or extensive strain variation.
URI: http://hdl.handle.net/10069/20112
ISSN: 00029637
PubMed ID: 17620639
関連リンク : http://www.ajtmh.org/cgi/content/abstract/77/1/107
権利: Copyright (c) 2007 by The American Society of Tropical Medicine and Hygiene
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:120 学術雑誌論文

引用URI : http://hdl.handle.net/10069/20112



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