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Field study on the distribution of Entamoeba histolytica and Entamoeba dispar in the northern Philippines as detected by the polymerase chain reaction


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タイトル: Field study on the distribution of Entamoeba histolytica and Entamoeba dispar in the northern Philippines as detected by the polymerase chain reaction
著者: Rivera, Windell L. / Tachibana, Hiroshi / Kanbara, Hiroji
発行日: 1998年12月
出版者: American Society of Tropical Medicine and Hygiene
引用: American Journal of Tropical Medicine and Hygiene, 59(6), pp.916-921; 1998
抄録: We used the polymerase chain reaction (PCR) to study the distribution of Entamoeba histolytica and E. dispar in 1,872 individuals in 14 communities in the northern Philippines. Here we report a field study using a DNA extraction protocol from formalin-fixed stool specimens as previously reported. This assay detected 137 stools (7.318%) containing E. dispar and 18 stools (0.961%) containing E. histolytica. The most affected age group for E. histolytica/E. dispar infections were those 5-14 years of age. There was no significant difference in the sex distribution of E. histolytica, while in the case of E. dispar, a higher prevalence was observed in females (9.186%) than in males (5.731%) (P < 0.01). An apparent clustering of stool-positive cases of E. histolytica and E. dispar was also observed in the northern part of the study area. The results of this survey demonstrate that E. dispar is highly prevalent in the communities studied. Moreover, it offers promise for the PCR using DNA extracted from formalin-fixed stools as a sensitive epidemiologic tool for detecting E. histolytica and E. dispar infections.
URI: http://hdl.handle.net/10069/20120
ISSN: 00029637
PubMed ID: 9886200
関連リンク : http://www.ajtmh.org/cgi/content/abstract/59/6/916
権利: Copyright (c) 1998 by The American Society of Tropical Medicine and Hygiene
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:120 学術雑誌論文

引用URI : http://hdl.handle.net/10069/20120

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