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In Vivo Anti-estrogenic Effects of Menadione on Hepatic Estrogen-responsive Gene Expression in Male Medaka (Oryzias latipes)

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Title: In Vivo Anti-estrogenic Effects of Menadione on Hepatic Estrogen-responsive Gene Expression in Male Medaka (Oryzias latipes)
Authors: Yamaguchi, Akemi / Kohra, Shinya / Ishibashi, Hiroshi / Arizono, Koji / Tominaga, Nobuaki
Issue Date: Oct-2008
Publisher: The Pharmaceutical Society of Japan
Citation: Journal of Health Science, 54(5), pp.596-601; 2008
Abstract: Menadione, a synthetic vitamin K3, exhibits anti-estrogenic activity on in vitro assay. However, the in vivo anti-estrogenic effects of menadione have not been determined, while correlations between biological effects and structural changes are unclear. Thus, we investigated the in vivo anti-estrogenic activity of menadione under fluorescent light and dark conditions. Suppression of the hepatic estrogen response genes vitellogenin1 (VTG1), VTG2 and estrogen receptor-α (ER-α) was used as an index of antiestrogenic activity. Male medaka (Oryzias latipes) were treated with nominal concentrations of menadione in the presence or absence of 17β-estradiol (E2), and hepatic VTG1, VTG2 and ER-α mRNA levels were determined by quantitative real-time PCR. In the presence of E2 under dark conditions, expression of hepatic VTG2 and ER-α genes was suppressed by menadione treatment. On the other hand, menadione activity was lost under fluorescent light conditions. These results suggest that menadione has antiestrogenic activity in vivo, and that this activity is diminished under fluorescent light, probably due to a structural change in menadione.
Keywords: menadione / anti-estrogenic / oryzias latipes, in vivo
URI: http://hdl.handle.net/10069/20173
ISSN: 13449702
DOI: 10.1248/jhs.54.596
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/20173

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