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蛍光検出高速液体クロマトグラフィーによるマウス脳内バルプロ酸の定量とその薬理学への応用


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Title: 蛍光検出高速液体クロマトグラフィーによるマウス脳内バルプロ酸の定量とその薬理学への応用
Other Titles: Fluorometric determination of valproic acid in mouse brain by HPLC and applications to pharmacology.
Authors: 松山, 賢治 / 宮崎, 長一郎 / 山下, 達也 / 市川, 正孝
Authors (alternative): Matsuyama, Kenji / Miyazaki, Choichiro / Yamashita, Tatsuya / Ichikawa, Masataka
Issue Date: 15-Nov-1989
Publisher: 社団法人日本分析化学会
Citation: 分析化学. 38(11), pp.T174-T178; 1989
Abstract: マウスに対してバルプロ酸(VPA)200mg/kgを腹くう内投与した後,HPLCを用いて脳内及び血中のVPA蛍光定量を行った.同時にVPA投与により引き起こされる脳内アミノ酸,4-アミノ酪酸(GABA),アスパラギン酸塩及びグルタミン酸の変化を検討した.その結果,VPAの抗てんかん作用は抑制性アミノ酸であるGABAの有意な上昇と同時に,興奮性アミノ酸であるアスパラギン酸とグルタミン酸の有意な減少に基づくものであることが実証された. / A fluorometric HPLC method for the determination of valproic acid (VPA) in mouse brain was developed using 3-bromomethyl-6, 7-dimethoxy-l-methyl-2 (1H) -quino-xalinone (Br-DMEQ) as a prelabelling reagent. The method was used to study the interrelations among the concentrations of VPA, 7-aminobutyrate as an inhibitory amino acid and glutamate or aspartate as an excitatory amino acid in mouse brain. The brain levels of VPA decreased with a half life of 0.99 h and attained almost zero at 5 h after the intraperitoneal administration of VPA at a dose of 200 mg/kg to mice. The administration of VPA gave rise to a significant increase in γ-aminobutyrate in response to a significant decrease in aspartate and glutamate in mouse brain. In particular, aspartate continued to decrease up to 8 h after administration whereas VPA disappeared at 5 h. The present observations are considered closely correlated to the "carried over effect" of VPA.
Description: <特集>バイオアナリティカルケミストリー
Keywords: fluorometric HPLC / valproic acid / glutamate / γ-aminobutyrate / aspartate / pharmacokinetics of valproic acid in the brain
URI: http://hdl.handle.net/10069/20212
ISSN: 05251931
Relational Links: http://ci.nii.ac.jp/naid/110002909907/
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/20212

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