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Selective determination of doxorubicin and doxorubicinol in rat plasma by HPLC with photosensitization reaction followed by chemiluminescence detection


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Title: Selective determination of doxorubicin and doxorubicinol in rat plasma by HPLC with photosensitization reaction followed by chemiluminescence detection
Authors: Ahmed, Sameh / Kishikawa, Naoya / Ohyama, Kaname / Wada, Mitsuhiro / Nakashima, Kenichiro / Kuroda, Naotaka
Issue Date: 15-Apr-2009
Publisher: Elsevier B.V.
Citation: Talanta, 78(1), pp.94-100; 2009
Abstract: A highly sensitive and selective high-performance liquid chromatography (HPLC) method was developed for the determination of doxorubicin (DXR) and its metabolite doxorubicinol (DXR-ol) in rat plasma. The method was based on photosensitization reaction followed by peroxyoxalate chemiluminescence detection (PO-CL). DXR and DXR-ol that were fluorescent quinones, served as a photosensitizer in the presence of a hydrogen atom donor such as ethanol under aerobic conditions to produce hydrogen peroxide. Then the generated hydrogen peroxide and DXR or DXR-ol were monitored through PO-CL reaction by mixing with aryloxalate as a single post-column reagent that enabled highly selective and sensitive determination of DXR and DXR-ol. The separation of DXR and DXR-ol by HPLC was accomplished isocratically on an ODS column within 15 min. The method involves a simple one step protein precipitation by methanol and a sample size of 50-μL was sufficient. Besides, it can detect accurately the low plasma concentrations. The detection limits (signal-to-noise ratio = 3) were 4.5 and 3.8 fmol for DXR and DXR-ol, respectively. The percentage recovery was found to be 90.7–102.4% and the inter- and intra-assay RSD values in rat plasma were 2.5–8.9%. The method has been successfully used to study pharmacokinetic profiles of DXR and DXR-ol in rats after a single-dose of DXR.
Keywords: Doxorubicin / Doxorubicinol / Pharmacokinetics / Peroxyoxalate chemiluminescence detection / Photosensitization
URI: http://hdl.handle.net/10069/20841
ISSN: 00399140
DOI: 10.1016/j.talanta.2008.10.043
Rights: Copyright © 2008 Elsevier B.V. All rights reserved.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/20841

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