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養殖マダイの氷蔵中における普通筋中 α-アクチニンの限定分解


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Title: 養殖マダイの氷蔵中における普通筋中 α-アクチニンの限定分解
Other Titles: Limitted Degradation of α-actinin in the Ordinary Muscle of Cultured Red Sea Bream during Storage in Ice
Authors: 橘, 勝康 / 鈴木, 秀一 / 八木, 基明 / 三嶋, 敏雄 / 原, 研治 / 槌本, 六良
Authors (alternative): Tachibana, Katsuyasu / Suzuki, Hidekazu / Yagi, Motoaki / Mishima, Tosio / Hara, Kenji / Tsuchimoto, Mutsuyosi
Issue Date: 2001
Publisher: 社団法人日本水産学会
Citation: 日本水産学会誌 = Bulletin of the Japanese Society of Scientific Fisheries. 2001, 67(4), p.723-727
Abstract: 養殖マダイ普通筋α-アクチニン抗体を作成し, これを用いて養殖マダイ氷蔵中における普通筋中α-アクチニンの分解をSDS-PAGEとイムノブロッティングで解析した。作成したα-アクチニン抗体はマダイ普通筋中のα-アクチニンのみならず, そのパパイン分解物とも反応性を示した。本抗体は免疫電顕による観察で, 筋原線維のZ線に特異的に反応し, 他の細胞内小器官等には反応を認めなかった。普通筋中α-アクチニンは氷蔵1日目から自己消化され, 5日目以降ではすべて分子量90kDa∿100kDaの成分に限定分解されていた。 / Anti-α-actinin IgG was prepared from rat serum immunized with purified α-actinin in the ordinary muscle of red sea bream. Anti-α-actinin IgG showed immune reactivity against not only α-actinin in ordinary muscle but also papain-digests of the α-actinin. According to immunoelectron microscopic method, α-actinin was detected on Z line of ordinary muscle cells and non-specific reactions were not detected on another organelles. The changes of α-actinin in the ordinary muscle of cultured red sea bream during storage in ice were examined by SDS-PAGE followed by its immunoblotting. It was found that the limited digestion of α-actinin in ordinary muscle of cultured red sea bream started on day one in ice, then, native α-actinin was not detected on day 5 and 7. These results suggested that the weakening of Z-disks in ordinary muscle was caused by the disappearance of α-actinin and resulted in the softness of the flesh texture of cultured red sea bream.
Keywords: α-アクチニン / 養殖マダイ / イムノブロッティング / 免疫電子顕微鏡 / Z線 / 筋原線維 / SDS-PAGE
URI: http://hdl.handle.net/10069/21551
ISSN: 00215392
Relational Links: http://ci.nii.ac.jp/naid/110003145329/
Rights: 社団法人日本水産学会 / 本文データは学協会の許諾に基づきCiNiiから複製したものである
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/21551

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