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キャピラリー電気クロマトグラフィー用新規機能性充填剤の開発(博士論文要録)


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Title: キャピラリー電気クロマトグラフィー用新規機能性充填剤の開発(博士論文要録)
Other Titles: Novel and Functional Stationary Phase for Capillary Electrochromatography(Digest of Doctoral Dissertation)
Authors: 大山, 要
Authors (alternative): Ohyama, Kaname
Issue Date: Jan-2007
Publisher: 日本分析化学会
Citation: 分析化学 56(1), p. 63-64 ; 2007
Abstract: Capillary electrochromatography (CEC) is a hybrid microseparation technique that combines electrophoretic and chromatographic separation principle. CEC has not yet found wide acceptance as a practical tool in the chromatographic community. This narrow currency of CEC is mainly attributed to two factors: 1) much fewer examples of real sample analysis by CEC than those by HPLC; 2) a limited variation of the stationay phase specially designed for CEC. In this thesis, CEC with NAIP, 3-(1,8-naphthalimido) propyl-modified silyl silica gel, demonstrated suitability for the prompt screening of toxic drugs and the expeditious analysis of microdialysate. Good repeatability of the proposed method with a durable NAIP column was proved to be sufficient for real sample analysis. Moreover, SNAIP, 3-(4-sulfo-1,8-naphthal-imido) propyl-modified silyl silica gel, was specially designed as a CEC stationary phase and, as expected, the sulfonic acid group in SNAIP worked as a strong EOF generator. In addition to hydrophobic and π-π interactions, an electrostatic interaction participated in the retention mechanism of SNAIP. This multiple retentive mechanism indicated an advantage in the separation of peptides, which are difficult to be separated solely based on a hydrophobic interaction, and to be eluted early without high buffer concentration. The remarkable tolerance of current in CEC with SNAIP accomplished the purpose that a high concentration of buffer in mobile phase could be used for the accelerated elution of multiple charged peptides. The tolerance also enabled the use of a highly aqueous mobile phase for the separation of polar nucleosides and nucleic acid bases on a SNAIP column.
Keywords: capillary electrochromatography / real sample analysis / novel stationary phase.
URI: http://hdl.handle.net/10069/21811
ISSN: 05251931
Relational Links: http://hdl.handle.net/10069/7172
Rights: (c) 2007 The Japan Society for Analytical Cemistry
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/21811

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