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A comprehensive study on the immunological reactivity of the Hsp90 molecular chaperone.


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タイトル: A comprehensive study on the immunological reactivity of the Hsp90 molecular chaperone.
著者: Kawano, Toshihiro / Kobayakawa, Takeshi / Fukuma, Yutaka / Yukitake, Hideharu / Kikuchi, Yuichiro / Shoji, Mikio / Nakayama, Koji / Mizuno, Akio / Takagi, Takashi / Nemoto, Takayuki K
発行日: 2004年11月
出版者: Nihon Seikagakkai
引用: Journal of biochemistry. 136(5), pp.711-722; 2004
抄録: Periodontitis is a chronic infectious disease, Porphyromonas gingivalis being the most implicated pathogen. In the present study, we investigated the role of P. gingivalis HtpG (PgHtpG), a bacterial ortholog of mammalian Hsp90, in the growth of P. gingivalis and also assessed the immunological cross-reactivity of the members of the Hsp90 family. Antiserum against rat liver Hsp90 potently reacted with yeast Hsp90, called Hsc82, and also weakly with human Hsp90 (hHsp90) and human mitochondrial paralog Trap1, but did not react with PgHtpG, Escherichia coli HtpG, or human endoplasmic reticulum paralog Grp94. Moreover, among 19 monoclonal antibodies raised against hHsp90, nine cross-reacted with yeast Hsc82, and one with human Grp94, but none bound to PgHtpG or E. coli HtpG. Among them, three mAbs that strongly reacted with yeast Hsc82 recognized Asn(291)-Ile(304), a conserved region of the family protein. The polyclonal antibody raised against a peptide, Met(315)-Glu(328), of human Grp94, which corresponded to the conserved region of hHsp90, cross-reacted with hHsp90, but not with other Hsp90-family members. Thus, although mammalian Hsp90 shares some immunological reactivity with yeast Hsc82, human Grp94, and human Trap1, it is considerably distinct from its bacterial ortholog, HtpG. Disruption of the P. gingivalis htpG gene neither affected bacterial survival nor altered the sensitivity of P. gingivalis to various forms of stress.
URI: http://hdl.handle.net/10069/21846
ISSN: 0021924X
DOI: 10.1093/jb/mvh178
PubMed ID: 15632312
関連リンク : http://jb.oxfordjournals.org/
権利: (c) 2004 The Japanese Biochemical Society / This is a pre-copy-editing, author-produced PDF of an article accepted for publication in "Journal of Biochemistry" following peer review. The definitive publisher-authenticated version "Journal of Biochemistry 2004 136(5):711-722" is available online at: http://jb.oxfordjournals.org/cgi/content/abstract/136/5/711
資料タイプ: Journal Article
原稿種類: author
出現コレクション:040 学術雑誌論文

引用URI : http://hdl.handle.net/10069/21846

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