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Two-dimensional gel electrophoresis analysis in simultaneous influenza pneumonia and bacterial infection in mice.


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タイトル: Two-dimensional gel electrophoresis analysis in simultaneous influenza pneumonia and bacterial infection in mice.
著者: Kosai, Kosuke / Seki, Masafumi / Yanagihara, Katsunori / Nakamura, Shigeki / Kurihara, Shintaro / Imamura, Yoshifumi / Izumikawa, Koichi / Kakeya, Hiroshi / Yamamoto, Yoshihiro / Tashiro, Takayoshi / Kohno, Shigeru
発行日: 2008年 5月
出版者: Wiley-Blackwell
引用: Clinical and Experimental Immunology, 152(2), pp.364-371; 2008
抄録: Severe pneumonia is found in simultaneous influenza pneumonia and bacterial infection, and suggests a relationship with immunological mechanisms. Here, we performed two-dimensional gel electrophoresis to detect immunological molecules related to the fulminant pneumonia caused by influenza virus and Streptococcus pneumoniae co-infection in mice. We found two spots that were expressed strongly in co-infected mouse lungs, compared with S. pneumoniae or influenza virus singly infected mouse lungs. The spots were analysed by mass spectrometry, and identified as alpha-1 anti-trypsin (A1AT), known as an anti-protease for neutrophil-derived proteolytic enzymes, and creatine kinase, which reflects a greater degree of lung damage and cell death. A1AT expression was increased significantly, and proteolytic enzymes from neutrophils, such as neutrophil elastase, myeloperoxidase and lysozyme, were also secreted abundantly in influenza virus and S. pneumoniae co-infected lungs compared with S. pneumoniae or influenza virus singly infected lungs. These data suggest that A1AT may play a central role as a molecule with broad anti-inflammatory properties, and regulation of the neutrophil-mediated severe lung inflammation is important in the pathogenesis of co-infection with influenza virus and bacteria.
キーワード: alpha-1 antitrypsin / co-infection / neutrophil elastase / myeloperoxidase / lysozyme
URI: http://hdl.handle.net/10069/21877
ISSN: 13652249
DOI: 10.1111/j.1365-2249.2008.03631.x
PubMed ID: 18341613
権利: Journal Compilation © 2008 British Society for Immunology / The definitive version is available at www.blackwell-synergy.com.
資料タイプ: Journal Article
原稿種類: author
出現コレクション:030 学術雑誌論文

引用URI : http://hdl.handle.net/10069/21877

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