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Detection of Type 1 Fimbrial Genes in Fish Pathogenic and Non-pathogenic Edwardsiella tarda Strains by PCR


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Title: Detection of Type 1 Fimbrial Genes in Fish Pathogenic and Non-pathogenic Edwardsiella tarda Strains by PCR
Other Titles: PCR法によるEdwardsiella tardaの病原性株および非病原性株からのtype1線毛遺伝子の検出
Authors: Sakai, Takamitsu / Iida, Takaji / Osatomi, Kiyoshi / Kanai, Kinya
Authors (alternative): 坂井, 貴光 / 飯田, 貴次 / 長富, 潔 / 金井, 欣也
Issue Date: 2007
Publisher: 日本魚病学会 / The Japanese Society of Fish Pathology
Citation: 魚病研究 = Fish Pathology 42(2) pp.115-117; 2007
Abstract: Presence of fimbrial genes among fish pathogenic and non-pathogenic strains of Edwardsiella tarda was investigated by polymerase chain reaction (PCR). Four primer sets for PCR were designed to detect the four genes (etfA, etfB, etfC, etfD) of the type 1 fimbrial gene cluster of E. tarda. All the four genes were successfully amplified with the four primer sets in all the pathogenic strains. On the other hand, any of etfA, etfB and etfC were not detected in 13 of 14 non-pathogenic strains. The results suggest that fimbriae play a role in the pathogenicity of E. tarda and that detection of etfA, etfB and etfC by PCR is useful to distinguish between pathogenic and non-pathogenic strains of E. tarda.
Keywords: Edwardsiella tarda / type 1 fimbrial gene / PCR / pathogenicity / edwardsiellosis
URI: http://hdl.handle.net/10069/21949
ISSN: 0388788X
DOI: 10.3147/jsfp.42.115
Rights: Copyright (c) 2007 The Japanese Society of Fish Pathology
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/21949

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