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The development of a gene vector electrostatically assembled with a polysaccharide capsule.


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Title: The development of a gene vector electrostatically assembled with a polysaccharide capsule.
Authors: Kurosaki, Tomoaki / Kitahara, Takashi / Kawakami, Shigeru / Nishida, Koyo / Nakamura, Junzo / Teshima, Mugen / Nakagawa, Hiroo / Kodama, Yukinobu / To, Hideto / Sasaki, Hitoshi
Issue Date: Sep-2009
Publisher: Elsevier
Citation: Biomaterials, 30(26), pp.4427-4434; 2009
Abstract: The purpose of this study was to develop a gene vector electrostatically assembled with a polysaccharide capsule. We used pDNA/polyethylenimine (PEI) complexes as efficient non-viral vectors. The pDNA/PEI complex was electrostatically encapsulated with various polysaccharides such as fucoidan, lambda-carrageenan, xanthan gum, alginic acid, hyaluronic acid, and chondroitin sulfate (CS). The pDNA/PEI complex was shown as nanoparticles with positive zeta-potential, although the ternary complexes encapsulated with polysaccharides were shown as nanoparticles with negative zeta-potential. The pDNA/PEI complex showed high agglutination activity and cytotoxicity, although the ternary complexes encapsulated with polysaccharides had no agglutination activities and lower cytotoxicities. The pDNA/PEI complex showed high uptake and high transgene efficiency in B16-F10 cells. On the other hand, most of the ternary complexes show little uptake and gene expression. The ternary complex encapsulated by CS, however, showed comparable transgene efficiency to the pDNA/PEI complex. The uptake and gene expression of the ternary complex encapsulated by CS were significantly inhibited by hypothermia and the addition of CS, suggesting that the ternary complex was taken by CS-specific receptor-mediated energy-dependent process.
Keywords: Self assembly / Gene transfer / Surface modification / DNA / Chondroitin sulfate
URI: http://hdl.handle.net/10069/21993
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2009.04.041
PubMed ID: 19473696
Rights: Copyright (c) 2009 Elsevier Inc. All rights reserved.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/21993

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