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Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol–biotin chemiluminescent polymer


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タイトル: Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol–biotin chemiluminescent polymer
著者: Zhang, Huan / Shibata, Takayuki / Krawczyk, Tomasz / Kabashima, Tsutomu / Lu, Jianzhong / Lee, Myung K. / Kai, Masaaki
発行日: 2009年 8月
出版者: Elsevier B.V.
引用: Talanta, 79(3), pp.700-705; 2009
抄録: Facile and non-radioactive methods are desired for the sensitive detection and quantification of various proteins. Herein we describe a novel chemiluminescence (CL)-detection method of particular proteins based on direct binding of a dextran-luminol-biotin (DLB) CL polymer to the proteins on a poly(vinylidene difluoride) membrane. Among 32 kinds of the proteins screened, several proteins such as drug-metabolizing enzymes, cytochrome p450 (CYP)1A2, CYP2E1, and CYP3A4 had the ability to bind directly to the DLB polymer. The binding site in the polymer was owing to the framework of the modified dextran, which underwent oxidation and reduction procedures. This interaction might be the comprehensive effect of both electrostatic interaction and steric complementarities. CL intensity of the proteins detected by the polymer could be further enlarged by the mediation of avidin. The proposed CL-imaging method possesses potential as a rapid, facile, inexpensive and selective detection of the proteins.
キーワード: Chemiluminescence / Luminol-containing macromolecule / Microchip / Protein detection
URI: http://hdl.handle.net/10069/22005
ISSN: 00399140
DOI: 10.1016/j.talanta.2009.04.056
権利: (c) 2009 Elsevier B.V. All rights reserved.
資料タイプ: Journal Article
原稿種類: author
出現コレクション:050 学術雑誌論文

引用URI : http://hdl.handle.net/10069/22005

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