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Alteration of p53-binding protein 1 expression during skin carcinogenesis: association with genomic instability.

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Title: Alteration of p53-binding protein 1 expression during skin carcinogenesis: association with genomic instability.
Authors: Naruke, Yuki / Nakashima, Masahiro / Suzuki, Keiji / Matsuu-Matsuyama, Mutsumi / Shichijo, Kazuko / Kondo, Hisayoshi / Sekine, Ichiro
Issue Date: May-2008
Citation: Cancer science, 99(5), pp.946-951; 2008
Abstract: Epidermal cells are the first cells to be exposed to environmental genotoxic agents such as ultraviolet (UV) and ionizing radiations (IR), which induce DNA double strand breaks (DSBs) and activate DNA damage response (DDR) to maintain genomic integrity. Defective DDR can result in genomic instability (GIN) which is considered to be a central aspect of any carcinogenic process. P53-binding protein 1 (53BP1) belongs to a family of evolutionarily conserved DDR proteins. Because 53BP1 molecules localize at the sites of DSBs and rapidly form nuclear foci, the presence of 53BP1 nuclear foci can be considered as a cytological marker for endogenous DSBs reflecting GIN. The levels of GIN were analyzed by immunofluorescence studies of 53BP1 in 56 skin tumors that included 20 seborrheic keratosis (SK), 8 actinic keratosis (AK), 9 Bowen’s disease (BD), 9 squamous cell carcinoma (SCC), and 10 basal cell carcinoma (BCC). This study demonstrated a number of nuclear 53BP1 foci in human skin tumorigenesis, suggesting a constitutive activation of DDR in skin cancer cells. Because AK showed a high DDR type of 53BP1 immunoreactivity, GIN seems to be induced at the precancerous stage. Furthermore, invasive cancers exhibited a high level of intense, abnormal 53BP1 nuclear staining with nuclear accumulation of p53, suggesting a disruption of DDR leading to a high level of GIN in cancer cells. The results of this study suggest that GIN has a crucial role in the progression of skin carcinogenesis. The detection of 53BP1 expression by immunofluorescence can be a useful histological marker to estimate the malignant potential of human skin tumors.
Keywords: skin cancer / genomic instabilit / 53BP1 / p53 / immunofluorescence
URI: http://hdl.handle.net/10069/22031
ISSN: 13479032
DOI: 10.1111/j.1349-7006.2008.00786.x
PubMed ID: 18380789
Rights: © 2008 Japanese Cancer Association / The definitive version is available at www.blackwell-synergy.com
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/22031

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